Yukioka K, Inaba M, Furumitsu Y, Yukioka M, Nishino T, Goto H, Nishizawa Y, Morii H
Yukioka Hospital, Second Department of Internal Medicine, Osaka City University Medical School, Japan.
J Rheumatol. 1994 Dec;21(12):2184-9.
To determine the levels of hepatocyte growth factor (HGF) in synovial fluids (SF) and sera from patients with rheumatoid arthritis (RA); to examine how these correlate with several disease variables in patients with RA and with levels of interleukin-6 (IL-6) in SF of these patients; and to examine whether HGF is released from adherent synovial cells (ASC) and synovial fluid cells (SF cells).
An enzyme linked immunosorbent assay was used to measure levels of HGF and IL-6. SF samples were obtained from 22 patients with RA, 12 with osteoarthritis (OA), and one with septic arthritis. Serum samples were collected from 40 patients with RA. HGF levels in culture supernatants from ASC and SF cells were measured.
The mean values of HGF in SF were 1.21 ng/ml for patients with RA, 0.19 ng/ml for those with OA and 0.18 ng/ml for the one with septic arthritis. HGF levels in SF of patients with RA were significantly higher than of those with OA (p < 0.01). The levels for patients with RA correlated with the serum C-reactive protein concentrations (r = 0.626, p < 0.01) and IL-6 levels in SF (r = 0.476, p < 0.05). The mean value of HGF in sera from patients with RA was 0.28 ng/ml. HGF levels in SF were higher than those in sera drawn simultaneously from the same patients with RA. In vitro, release of HGF from rheumatoid ASC was not detected. However, SF cells from patients with RA released HGF spontaneously.
Our observations suggest that HGF in SF of patients with RA is produced by SF cells and is related to disease activity of RA, and thus that HGF may play a role in RA.
测定类风湿关节炎（RA）患者滑液（SF）和血清中肝细胞生长因子（HGF）的水平；研究这些水平与RA患者的几个疾病变量以及这些患者SF中白细胞介素-6（IL-6）水平之间的相关性；并研究HGF是否由贴壁滑膜细胞（ASC）和滑液细胞（SF细胞）释放。
采用酶联免疫吸附测定法测量HGF和IL-6的水平。从22例RA患者、12例骨关节炎（OA）患者和1例化脓性关节炎患者中获取SF样本。从40例RA患者中采集血清样本。测量ASC和SF细胞培养上清液中的HGF水平。
RA患者SF中HGF的平均值为1.21 ng/ml，OA患者为0.19 ng/ml，化脓性关节炎患者为0.18 ng/ml。RA患者SF中的HGF水平显著高于OA患者（p < 0.01）。RA患者的HGF水平与血清C反应蛋白浓度（r = 0.626，p < 0.01）和SF中的IL-6水平（r = 0.476，p < 0.05）相关。RA患者血清中HGF的平均值为0.28 ng/ml。RA患者SF中的HGF水平高于同时从同一患者采集的血清中的水平。在体外，未检测到类风湿ASC释放HGF。然而，RA患者的SF细胞自发释放HGF。
我们的观察结果表明，RA患者SF中的HGF由SF细胞产生，并且与RA的疾病活动相关，因此HGF可能在RA中起作用。
