长链非编码RNA HOTAIR mRNA在卵巢癌中的表达
[Expression of long non-coding RNA HOTAIR mRNA in ovarian cancer].
作者信息
Cui Ling, Xie Xiao-Yan, Wang He, Chen Xin-Lian, Liu Shan-Ling, Hu Li-Na
机构信息
Laboratory of Genetics, West China Institute of Women and Children's Health, West China Second Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 Jan;44(1):57-9.
OBJECTIVE
To investigate the long non-coding RNA HOTAIR (long non-coding HOX antisense intergenic RNA) by examining HOTAIR expression levels in ovarian cancer tissue and normal ovarian tissue.
METHODS
The mRNA of long non-coding RNA HOTAIR expressions were detected by real-time fluorescence quantitative PCR in ovarian cancer (44) and normal ovary tissues (14).
RESULTS
The expression of HOTAIR in ovarian cancer tissue was higher than that in normal ovarian tissue (1.26 +/- 0.27 vs. 0.14 +/- 0.09, P < 0.05). The expression was statistically higher in poorly differentiated ovarian cancer than poorly-moderately, moderately-well, and well-differentiated ones (1.65 +/- 0.41 vs. 0.39 +/- 0.14, P < 0.05).
CONCLUSION
RNA HOTAIR expressions were higher in ovarian cancer; it may play a role in ovarian cancer, and become a biomarker for malignant degree of ovarian cancer and may provide a novel therapeutic target for ovarian cancer.
目的
通过检测卵巢癌组织和正常卵巢组织中长链非编码RNA HOTAIR（长链非编码HOX反义基因间RNA）的表达水平，对其进行研究。
方法
采用实时荧光定量PCR检测44例卵巢癌组织和14例正常卵巢组织中长链非编码RNA HOTAIR的mRNA表达。
结果
卵巢癌组织中HOTAIR的表达高于正常卵巢组织（1.26±0.27对0.14±0.09，P<0.05）。在低分化卵巢癌中的表达显著高于中低分化、中高分化和高分化卵巢癌（1.65±0.41对0.39±0.14，P<0.05）。
结论
RNA HOTAIR在卵巢癌中表达较高；它可能在卵巢癌中发挥作用，成为卵巢癌恶性程度的生物标志物，并可能为卵巢癌提供新的治疗靶点。
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