Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70808, USA.
J Vet Intern Med. 2013 May-Jun;27(3):456-61. doi: 10.1111/jvim.12085. Epub 2013 Apr 18.
Limited data exist regarding influence of endoscopic forceps on duodenal mucosal biopsy quality and adequacy for histologic examination/assessment in dogs.
HYPOTHESIS/OBJECTIVES: Hypothesizing that larger forceps would procure superior specimens, we evaluated effect of 6 disposable forceps on duodenal biopsy weight, depth, crush artifact, and adequacy for histologic examination/assessment.
Seventeen healthy adult dogs.
Prospective study. Two operators each obtained 4 duodenal specimens from each dog with each forceps. Lightest sample discarded. One pathologist evaluated blindly other 3 specimens. A total of 612 specimens evaluated. Results analyzed by one-way ANOVA of forceps effects with dog as blocking factor. Posthoc pairwise comparisons examined with Tukey's test when indicated.
Biopsies performed with large capacity forceps heavier (10.56 ± 0.90 and 11.6 ± 0.62 mg (mean ± SD) versus 5.55 ± 0.53 to 8.61 ± 0.49; P < .0001) and adequacy for histologic examination/assessment superior to standard oval and 'pediatric' (scores 2.52 ± 0.41 and 2.58 ± 0.37 versus 2.08 ± 0.33 and 2.14 ± 0.29; P < .0001). No statistically significant difference in depth scores. Large capacity forceps with spike associated with less crush artifact than all smaller forceps (scores 1.19 ± 0.16 versus 1.38 ± 0.21 to 1.52 ± 0.21; P < .0001). In same size forceps, presence of spike had no effect on crush artifact and adequacy for histologic examination/assessment (P < .0001).
Large capacity forceps are superior, providing higher quality and greater numbers of samples achieving adequacy for histologic examination/assessment. Choice of endoscopic biopsy forceps for duodenal samples influences sample volume and diagnostic utility.
有关内镜活检钳对犬十二指肠黏膜活检质量和组织学评估适用性的影响，数据有限。
假设/目的：假设较大的活检钳可获得更好的标本，我们评估了 6 种一次性活检钳对十二指肠活检重量、深度、压碎伪影和组织学评估适用性的影响。
17 只健康成年犬。
前瞻性研究。两位操作者使用每种活检钳从每只犬获取 4 份十二指肠标本。丢弃最轻的样本。一位病理学家对其余 3 个标本进行盲法评估。共评估了 612 个标本。结果采用单因素方差分析（以犬为分层因素）进行分析。当需要时，采用 Tukey 检验进行事后两两比较。
大容量活检钳采集的标本重量更重（10.56 ± 0.90 和 11.6 ± 0.62mg（平均值 ± SD）与 5.55 ± 0.53 至 8.61 ± 0.49；P <.0001），且组织学评估适用性优于标准椭圆形和“儿科”活检钳（评分 2.52 ± 0.41 和 2.58 ± 0.37 与 2.08 ± 0.33 和 2.14 ± 0.29；P <.0001）。深度评分无统计学差异。带刺的大容量活检钳比所有较小的活检钳压碎伪影更少（评分 1.19 ± 0.16 与 1.38 ± 0.21 至 1.52 ± 0.21；P <.0001）。在相同尺寸的活检钳中，带刺对压碎伪影和组织学评估适用性无影响（P <.0001）。
大容量活检钳更优，可提供更高质量和更多数量的标本，达到组织学评估适用性。选择用于十二指肠样本的内镜活检钳会影响样本量和诊断效用。
