吞噬作用期间多形核白细胞代谢刺激机制的研究。I. 超氧阴离子参与NADPH2氧化的证据。

The oxidation of NADPH2 by leucocyte granules, as measured at acid pH in the presence of Mn-2+, was found to be inhibited by superoxide dismutase. 2. Omission of Mn-2+ markedly lowered the oxidase activity at acid pH, which was still inhibited by superoxide dismutase. 3. At alkaline pH the oxidase activity was lower than at acid pH. 4. During oxidation of NADPH2 by leucocyte granules, reduction of cytochrome c occurred which was partially inhibited by superoxide dismutase. 5. It was concluded that NADPH2 oxidation occurs through an enzymatic reaction and a nonenzymatic chain reaction. Superoxide anion (O-minus-2 and NADPH- free radical would be involved in the chain reaction. The differential sensitivity of NADPH2 oxidation to superoxide dismutase in different experimental conditions (see above 1, 2 and 3) was explained on the basis of changes in the properties of the chain reaction.

在酸性pH值且存在锰离子（Mn-2+）的条件下测定，白细胞颗粒对还原型辅酶II（NADPH2）的氧化作用被超氧化物歧化酶抑制。2. 省略锰离子（Mn-2+）会显著降低酸性pH值下的氧化酶活性，而超氧化物歧化酶仍会抑制该活性。3. 在碱性pH值下，氧化酶活性低于酸性pH值时的活性。4. 在白细胞颗粒对还原型辅酶II（NADPH2）的氧化过程中，细胞色素c发生还原反应，该反应部分被超氧化物歧化酶抑制。5. 得出的结论是，还原型辅酶II（NADPH2）的氧化通过酶促反应和非酶链式反应发生。超氧阴离子（O-2-）和无还原型辅酶II（NADPH）自由基会参与链式反应。根据链式反应性质的变化，解释了还原型辅酶II（NADPH2）氧化在不同实验条件下（见上述1、2和3）对超氧化物歧化酶的不同敏感性。

Studies on the mechanism of metabolic stimulation in polymorphonuclear leucocytes during phagocytosis. I. Evidence for superoxide anion involvement in the oxidation of NADPH2.