槐耳浸膏通过 MEK/ERK 信号通路诱导肝癌细胞凋亡。
Huaier Extract Induces Apoptosis in Hepatoblastoma Cells the MEK/ERK Signaling Pathway.
机构信息
Department of Pediatric Hematology/Oncology, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China.
Department of Pediatrics, Mie University Graduate School of Medicine, Mie, Japan.
出版信息
In Vivo. 2020 Sep-Oct;34(5):2381-2388. doi: 10.21873/invivo.12051.
BACKGROUND/AIM: Huaier extract, whose main active constituent is the proteoglycan, has anti-tumor activity in several types of malignancies. In this study, we aimed to elucidate the effect of Huaier extract in hepatoblastoma cells.
MATERIALS AND METHODS
The effect of Huaier extract on the proliferation of human hepatoblastoma cell lines HepG2 and HuH-6, was examined.
RESULTS
Incubation with Huaier extract resulted in a marked, dose-dependent decrease in hepatoblastoma cell viability. Huaier extract induced S phase arrest in hepatoblastoma cells and upregulated the expression of the cell cycle related proteins cyclin D1 and cyclin D3. It also induced apoptosis in hepatoblastoma cells. Additionally, it significantly suppressed the activity of p-ERK and p-MEK.
CONCLUSION
Huaier extract inhibits proliferation, and induces cell apoptosis and cell cycle arrest via the MEK-ERK pathway in hepatoblastoma cells. Huaier extract may act as a complementary agent for treating hepatoblastoma.
背景/目的：白花蛇舌草提取物的主要活性成分为蛋白聚糖，在多种恶性肿瘤中具有抗肿瘤活性。在这项研究中，我们旨在阐明白花蛇舌草提取物对肝癌细胞的影响。
材料和方法
检测了白花蛇舌草提取物对人肝癌细胞系 HepG2 和 HuH-6 增殖的影响。
结果
白花蛇舌草提取物孵育导致肝癌细胞活力明显、剂量依赖性下降。白花蛇舌草提取物诱导肝癌细胞 S 期停滞，并上调细胞周期相关蛋白 cyclin D1 和 cyclin D3 的表达。它还诱导肝癌细胞凋亡。此外，它显著抑制 p-ERK 和 p-MEK 的活性。
结论
白花蛇舌草提取物通过 MEK-ERK 通路抑制肝癌细胞增殖，并诱导细胞凋亡和细胞周期停滞。白花蛇舌草提取物可能作为治疗肝癌的辅助药物。
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