Institut des Biomolécules Max Mousseron (IBMM), Artificial Biopolymers Group, UMR CNRS 5247, University of Montpellier 1, University of Montpellier 2, Faculty of Pharmacy, 15 Av. C. Flahault, Montpellier 34093, France.
Acta Biomater. 2013 Aug;9(8):7709-18. doi: 10.1016/j.actbio.2013.04.018. Epub 2013 Apr 17.
The effects of biomaterials on their environment must be carefully modulated in most biomedical applications. Among other approaches, this modulation can be obtained through the modification of the biomaterial surface. This paper proposes a simple and versatile strategy to produce non-leaching antibacterial polylactide (PLA) surfaces without any degradation of the polyester chains. The method is based on a one-pot procedure that provides a "clickable" PLA surface via anionic activation which is then functionalized with an antibacterial quaternized poly(2-(dimethylamino)ethyl methacrylate) (QPDMAEMA) by covalent immobilization on the surface. The anti-adherence and antibiofilm activities of modified PLA surfaces are assessed for different QPDMAEMA molecular weights and different quaternization agents. Antibacterial PLA surfaces are shown to be very active against Gram-negative and Gram-positive strains, with adherence reduction factors superior to 99.999% and a marked reduction in biofilm on the most potent surfaces. In addition to this substantial antibacterial activity, the proposed PLA surfaces are also cytocompatible, as demonstrated through the proliferation of L929 fibroblasts.
在大多数生物医学应用中，必须仔细调节生物材料对其环境的影响。除其他方法外，这种调节可以通过修饰生物材料表面来实现。本文提出了一种简单而通用的策略，可在不降解聚酯链的情况下制备无浸出的抗菌聚乳酸（PLA）表面。该方法基于一锅法，通过阴离子活化提供了一种“点击”PLA 表面，然后通过共价固定化在表面上用抗菌季铵化聚（2-（二甲氨基）乙基甲基丙烯酸酯）（QPDMAEMA）进行功能化。评估了不同 QPDMAEMA 分子量和不同季铵化剂对改性 PLA 表面的抗附着和抗生物膜活性。结果表明，抗菌 PLA 表面对革兰氏阴性和革兰氏阳性菌株非常有效，附着减少因子优于 99.999%，在最有效的表面上生物膜明显减少。除了这种强大的抗菌活性外，所提出的 PLA 表面还具有细胞相容性，这通过 L929 成纤维细胞的增殖得到了证明。
