Goßmann M, Beran F, Bedlan G, Plenk A, Hamedinger S, Ohlinger R, Humpf H-U, Büttner C
Fachgebiet Phytomedizin, Humboldt Universität zu Berlin, Institut für Gartenbau, Lentzeallee 55-57, 14195, Berlin, Germany,
Mycotoxin Res. 2008 Jun;24(2):88-97. doi: 10.1007/BF02985286.
Asparagus spears collected from a total of six commercial plantings in Austria during the main harvest periods in May and June of 2003 and 2004 were examined for endophytic colonization byFusarium spp., particularlyF. proliferatum. Potentially toxigenic fungi such asF. proliferatum were isolated and identified by morphological characteristics using light microscopy. Fumonisin B1 inF. proliferatum-infected asparagus spears was detected with IAS-HPLC-FLD or HPLC-MS/MS. The identity of endophytic fungi colonizing of a total of 816 individual spears was determined. The incidence of infection byF. proliferatum and otherFusarium spp. was highly dependent on location and sampling date. The dominantFusarium species among the endophytic microflora wasF. oxysporum. Other frequently isolated species includedF. proliferatum, F. sambucinum, F. culmorum, F. avenaceum andF. equiseti. The incidence ofF. proliferatum-infected asparagus spears was less than 10% at four of the six sampling locations. At the two remaining locations, 20-47% of the spears examined were infected withF. proliferatum. Further exploration of FB1 generation in asparagus is required because the low levels of FB1 (10-50 (μg/kg) detected in harvested spears in 2003 and 2004 cannot be explained by the results of this study.
在2003年和2004年5月及6月的主要收获期，从奥地利总共六个商业种植地采集芦笋嫩茎，检测其中镰刀菌属，特别是轮枝镰刀菌的内生定殖情况。利用光学显微镜通过形态特征分离并鉴定潜在产毒真菌，如轮枝镰刀菌。采用免疫亲和净化-高效液相色谱-荧光检测法（IAS-HPLC-FLD）或高效液相色谱-串联质谱法（HPLC-MS/MS）检测轮枝镰刀菌感染的芦笋嫩茎中的伏马毒素B1。确定了总共816根单株嫩茎上定殖的内生真菌种类。轮枝镰刀菌和其他镰刀菌属的感染发生率高度依赖于地点和采样日期。内生微生物群落中占主导地位的镰刀菌种类是尖孢镰刀菌。其他经常分离出的种类包括轮枝镰刀菌、接骨木镰刀菌、禾谷镰刀菌、燕麦镰刀菌和木贼镰刀菌。在六个采样地点中的四个地点，轮枝镰刀菌感染的芦笋嫩茎发生率低于10%。在其余两个地点，检测的嫩茎中有20%-47%被轮枝镰刀菌感染。由于2003年和2004年收获的嫩茎中检测到的低水平伏马毒素B1（10-50μg/kg）无法用本研究结果解释，因此需要进一步探索芦笋中伏马毒素B1的产生情况。
