高效液相色谱法分析单端孢霉烯族化合物——禾谷镰刀菌醇、新茄病镰刀菌烯醇和新茄病镰刀菌酮的分离。

HPLC of trichothecenes - Separation of neosolaniol, NT-1 toxin, and NT-2 toxin.

机构信息

Firma Hans W. Schmidt, Saarstr. 52, D-6500 Mainz 1, Federal Republic of Germany.

出版信息

Mycotoxin Res. 1986 Mar;2(1):39-43. doi: 10.1007/BF03191961.

DOI:10.1007/BF03191961

Abstract

A high pressure liquid chromatographic (HPLC) method is described for the isolation of trichothecenes formed on moldy rice. Extraction of the cultures was followed by purification and fractionation with a C18-Sep Pak cartridge. The polar fraction contained neosolaniol, 4,8-diacetoxy-12,13-epoxytrichothec-9-ene-3,15-diol (NT-1) and 4-acetoxy-12,13-epoxytrichothec-9ene-3,8,15-triol (NT-2), while in another fraction HT-2 toxin, T-2 toxin and acetyl-T-2 toxin were eluted. A high pressure liquid chromatograph equipped with a C18 μ Bondapak column and an R I-detector (differential refractometer) were used for the isolation procedure.

摘要

描述了一种高压液相色谱（HPLC）方法，用于分离霉变大米中形成的单端孢霉烯族化合物。培养物的提取后，用 C18-Sep Pak 小柱进行纯化和分离。极性馏分含有新茄病镰刀菌醇、4,8-二乙酰氧基-12,13-环氧单端孢霉-9-烯-3,15-二醇（NT-1）和 4-乙酰氧基-12,13-环氧单端孢霉-9-烯-3,8,15-三醇（NT-2），而另一个馏分中则洗脱了 HT-2 毒素、T-2 毒素和乙酰-T-2 毒素。使用配备 C18 μ Bondapak 柱和 RI 检测器（差示折射仪）的高压液相色谱仪进行分离程序。