高效液相色谱法：一种用于分析谷物中T-2毒素和HT-2毒素的工具。

HPLC: a tool for the analysis of T-2 toxin and HT-2 toxin in cereals.

作者信息

Schmidt R, Dose K

出版信息

J Anal Toxicol. 1984 Jan-Feb;8(1):43-5. doi: 10.1093/jat/8.1.43.

DOI:10.1093/jat/8.1.43

Abstract

An analytical procedure for the determination of trichothecenes in various cereals is described. HPLC was performed with a reversed-phase (C18) column eluted with methanol:water (60:40, v/v). Compounds were detected with a refractive index detector. The elution patterns of free and contaminated samples were compared. The recovery of added T-2 toxin (2 and 5 micrograms/g) in rye and wheat was approximately 80%. The application of this method allows for combined use with other sensitive methods such as mass spectrometry and gas chromatography. The described method is operationally simple, relatively inexpensive, and requires no derivatization.

摘要

本文描述了一种测定各种谷物中单端孢霉烯族毒素的分析方法。采用反相（C18）柱进行高效液相色谱分析，以甲醇∶水（60∶40，v/v）作为洗脱剂。使用折光率检测器检测化合物。比较了游离样品和受污染样品的洗脱模式。在黑麦和小麦中添加的T-2毒素（2和5微克/克）的回收率约为80%。该方法可与质谱和气相色谱等其他灵敏方法联合使用。所描述的方法操作简单、成本相对较低，且无需衍生化。