短小杆菌属菌株114 - 2中T - 2毒素的代谢
Metabolism of T-2 toxin in Curtobacterium sp. strain 114-2.
作者信息
Ueno Y, Nakayama K, Ishii K, Tashiro F, Minoda Y, Omori T, Komagata K
出版信息
Appl Environ Microbiol. 1983 Jul;46(1):120-7. doi: 10.1128/aem.46.1.120-127.1983.
Abstract
The metabolic pathway of T-2 toxin in Curtobacterium sp. strain 114, one of the T-2 toxin-assimilating soil bacteria, was investigated by thin-layer and gas-liquid chromatographic analyses. T-2 toxin added to the basal medium as a single carbon and energy source was biotransformed into HT-2 toxin and an unknown metabolite. Infrared, mass spectrum, proton magnetic resonance, and other physico-chemical analyses identified this new metabolite as T-2 triol. T-2 toxin was first deacetylated by the bacterium into HT-2 toxin, and this metabolite was then biotransformed into T-2 triol without formation of neosolaniol and T-2 tetraol. No trichothecenes remained in the culture medium after prolonged culture. Some properties of T-2 toxin-hydrolyzing enzymes were observed with whole cells, the cell-free soluble fraction, and the culture filtrate. Besides T-2 toxin, trichothecenes such as diacetoxyscirpenol, neosolaniol, nivalenol, and fusarenon-X were also assimilated by this bacterium.
摘要
对食酸菌属菌株114（一种能同化T-2毒素的土壤细菌）中T-2毒素的代谢途径进行了薄层色谱和气液色谱分析研究。添加到基础培养基中作为单一碳源和能源的T-2毒素被生物转化为HT-2毒素和一种未知代谢物。通过红外光谱、质谱、质子磁共振和其他物理化学分析确定这种新的代谢物为T-2三醇。T-2毒素首先被该细菌脱乙酰基转化为HT-2毒素，然后这种代谢物被生物转化为T-2三醇，未形成新茄病镰刀菌烯醇和T-2四醇。长时间培养后，培养基中未残留任何单端孢霉烯族毒素。利用完整细胞、无细胞可溶性部分和培养滤液观察了T-2毒素水解酶的一些特性。除T-2毒素外，该细菌还能同化双乙酰氧基镰孢菌烯醇、新茄病镰刀菌烯醇、雪腐镰刀菌烯醇和镰刀菌烯酮-X等单端孢霉烯族毒素。
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