Department of Biochemistry, University College of Science, Osmania University, Hyderabad, 500 007, Andhra Pradesh, India.
Mycotoxin Res. 2010 Aug;26(3):155-70. doi: 10.1007/s12550-010-0050-y. Epub 2010 Mar 24.
The effect of surfactants (two cationic, one anionic and three non-ionic) at 0.001, 0.01, 0.1 and 1.0 % concentrations on aflatoxin production, ergosterol content and sugar consumption by Aspergillus parasiticus (NRRL 2999) in YES liquid culture medium is reported. At 0.01% concentration, the cationic surfactants, cetyl dimethyl ammonium bromide (CDAB) and dodecyl trimethyl ammonium bromide (DTAB), and the anionic surfactant, sodium dodecyl sulfate (SDS), completely inhibited spore germination, while DTAB also inhibited the production of ergosterol and toxin (p < 0.05). At a concentration of 0.001%, CDAB was found to enhance toxin production, while SDS was found to inhibit it when compared with other surfactants. Non-ionic surfactants, polyoxyethylene sorbitan monolaurate (Tween-20), polyoxyethylene lauryl ether (Brij-35) and ethoxylated p-tert-octylphenol (Triton X-100) delayed the spore germination up to day 5 at all concentrations and inhibited toxin and ergosterol production at 0.001% concentration. The affect was found to be dose-dependent from 0.001% to 1%, for Triton X-100 only. Positive correlation between ergosterol content and toxin production in the presence of different surfactants at various time periods (3, 5, 7, 9 and 12 days) was found. Tween-20 was most effective in inhibiting toxin production on day 7, when aflatoxin production was found to be maximal in control group. Sugar consumption was directly proportional to the ergosterol content, showing a significant correlation with aflatoxin production.
本文报道了表面活性剂（两种阳离子型、一种阴离子型和三种非离子型）在 0.001%、0.01%、0.1%和 1.0%浓度下对寄生曲霉（NRRL 2999）在 YES 液体培养基中产生黄曲霉毒素、麦角固醇含量和糖消耗的影响。在 0.01%浓度下，阳离子表面活性剂十六烷基二甲基溴化铵（CDAB）和十二烷基三甲基溴化铵（DTAB）以及阴离子表面活性剂十二烷基硫酸钠（SDS）完全抑制了孢子萌发，而 DTAB 还抑制了麦角固醇和毒素的产生（p<0.05）。在 0.001%浓度下，发现 CDAB 促进了毒素的产生，而 SDS 与其他表面活性剂相比抑制了毒素的产生。非离子表面活性剂聚氧乙烯失水山梨醇单月桂酸酯（Tween-20）、聚氧乙烯月桂醚（Brij-35）和乙氧基化对叔辛基苯酚（Triton X-100）在所有浓度下均延迟了孢子萌发，直到第 5 天，并在 0.001%浓度下抑制了毒素和麦角固醇的产生。仅在 Triton X-100 中，从 0.001%到 1%的浓度，发现这种影响呈剂量依赖性。在不同时期（3、5、7、9 和 12 天）存在不同表面活性剂时，麦角固醇含量与毒素产生之间存在正相关关系。Tween-20 在第 7 天抑制毒素产生的效果最为明显，此时对照组中黄曲霉毒素的产生达到最大值。糖消耗与麦角固醇含量成正比，与黄曲霉毒素的产生呈显著相关。
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