Appl Environ Microbiol. 1997 Mar;63(3):1048-53. doi: 10.1128/aem.63.3.1048-1053.1997.
An experiment with a full factorial design was used to study the effects of and interactions among temperature, water activity (a(infw)), incubation period, and substrate on coproduction of aflatoxins (AF) and cyclopiazonic acid (CPA) by an isolate of Aspergillus flavus. Analysis of variance showed that there was a complex interaction among all of these factors and that this influenced the relative concentrations of the mycotoxins produced. The optimum temperatures for the production of AF and CPA were 30(deg)C and 25(deg)C, respectively. Both mycotoxins were maximally produced (0.306 to 0.330 (mu)g of AF(middot)ml of medium(sup-1), 4.040 to 6.256 (mu)g of CPA(middot)ml of medium(sup-1)) at an a(infw) of 0.996 and after 15 days of incubation. No AF were produced in either yeast extract agar or Czapek yeast autolysate agar medium at an a(infw) of 0.90 at 20 or 37(deg)C after 15 days (minimum conditions), while 0.077 to 0.439 (mu)g of CPA(middot)ml of medium(sup-1) was produced under the same conditions. Yeast extract agar favored maximum AF production, and Czapek yeast autolysate agar favored maximum CPA production.
采用完全析因设计实验来研究温度、水分活度（aw）、培养期和基质对黄曲霉（Aspergillus flavus）分离株同时产生黄曲霉毒素（AF）和环匹阿尼酸（CPA）的影响及其相互作用。方差分析表明，所有这些因素之间存在复杂的相互作用，这会影响所产生的霉菌毒素的相对浓度。AF 和 CPA 的最佳生产温度分别为 30℃和 25℃。在 aw 为 0.996 和培养 15 天后，两种霉菌毒素的产量最高（0.306 至 0.330μgAF/ml 培养基，4.040 至 6.256μgCPA/ml 培养基）。在 aw 为 0.90 时，在 20℃或 37℃下，无论是在酵母提取物琼脂还是在察氏酵母自溶物琼脂培养基中，15 天后均未产生 AF（最低条件），而在相同条件下则产生了 0.077 至 0.439μgCPA/ml 培养基。酵母提取物琼脂有利于 AF 的最大产量，而察氏酵母自溶物琼脂有利于 CPA 的最大产量。
