Pardo AG
Laboratorio de Micologia Experimental, Departamento de Ciencias Biologicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II 4degrees Piso, 1428-Buenos Aires, Argentine
Curr Microbiol. 1996 Oct;33(4):275-8. doi: 10.1007/s002849900113.
The effect of different nonionic surfactants (Tween 80, Tween 20, Triton X-100) and polyethylene glycol (PEG 6000) was tested on cellulolytic enzyme system production. Tween 80 gave the highest yield of endoglucanase, exoglucanase, and cellobiase at the 20th day of growth, presumably by causing increased permeability of cell membranes and/or by promoting the release of cell-bound enzymes. Maximal yield of endoglucanase was achieved with 1.7 mM Tween 80, whereas exoglucanase and cellobiase were at 0.85 mM. In the same way, this compound increased fungal growth. On the other hand, Tween 20 and Triton X-100 inhibited growth and cellulolytic enzyme production. High yields of endoglucanase and exoglucanase were achieved with PEG 6000 in comparison with the control, presumably by increasing enzyme stability.
测试了不同的非离子表面活性剂（吐温80、吐温20、曲拉通X-100）和聚乙二醇（PEG 6000）对纤维素分解酶系统产生的影响。在生长第20天时，吐温80使内切葡聚糖酶、外切葡聚糖酶和纤维二糖酶的产量最高，这可能是通过增加细胞膜的通透性和/或促进细胞结合酶的释放来实现的。用1.7 mM吐温80可实现内切葡聚糖酶的最大产量，而外切葡聚糖酶和纤维二糖酶的最大产量则在0.85 mM时实现。同样，该化合物促进了真菌生长。另一方面，吐温20和曲拉通X-100抑制了生长和纤维素分解酶的产生。与对照相比，PEG 6000实现了内切葡聚糖酶和外切葡聚糖酶的高产，这可能是通过提高酶的稳定性来实现的。
