玉米赤霉烯酮、脱氧雪腐镰刀菌烯醇和 15-乙酰脱氧雪腐镰刀菌烯醇的生物合成、分离、纯化和提取。
Biosynthesis, isolation, purification and seperation of Zearalenone, deoxynivalenol and 15-Acetyldeoxynivalenol.
机构信息
Department of Chemistry, Agricultural University of Poznań, ul. Wojska Polskiego 75, 60-625, Poznań, Poland.
出版信息
Mycotoxin Res. 1991 Sep;7 Suppl 2:156-9. doi: 10.1007/BF03192202.
Fusarium graminearum KF-376 isolate was found to be able to form simultaneously three toxic metabolites: zearalenone (FF-2), deoxynivalenol (DON) and 15-acetyldeoxynivalenol (15-AcDON). Toxins were extracted with methanol - water 3:1 (v/v) and purified by liquid chromatography on charcoal - Kieselgel 60 column (preliminary) and Aluminiumoxid 90 column. Final separation of the metabolites was achived on Kieselgel 60 - Aluminiumoxid 90 column.
镰刀菌 KF-376 分离株被发现能够同时形成三种有毒代谢物：玉米赤霉烯酮（FF-2）、脱氧雪腐镰刀菌烯醇（DON）和 15-乙酰脱氧雪腐镰刀菌烯醇（15-AcDON）。毒素用甲醇-水 3:1（v/v）提取，并用活性炭-硅胶 60 柱（初步）和氧化铝 90 柱进行液相色谱法纯化。最终代谢物的分离是在硅胶 60-氧化铝 90 柱上实现的。
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