从接种禾谷镰刀菌的大米中分离脱氧雪腐镰刀菌烯醇的简易方法。
Simple method for isolation of 4-deoxynivalenol from rice inoculated with Fusarium graminearum.
作者信息
Ehrlich K C, Lillehoj E B
出版信息
Appl Environ Microbiol. 1984 Nov;48(5):1053-4. doi: 10.1128/aem.48.5.1053-1054.1984.
Abstract
A new method for preparative isolation of 4-deoxynivalenol (DON) is presented. This method avoids the loss of material during purification on silica gel by column chromatography. DON and 3-acetyldeoxynivalenol in crude extracts of rice inoculated with Fusarium graminearum were converted to triacetyldeoxynivalenol; the acetylated product was easier to purify by silica gel chromatography than DON is. After hydrolysis and further purification on a charcoal-alumina column, the 71% pure DON was recovered in yields as high as 450 mg of DON per kg of rice. Subsequent separation on a Sephadex LH20 column yielded DON that was greater than 90% pure.
摘要
本文介绍了一种制备性分离4-脱氧雪腐镰刀菌烯醇（DON）的新方法。该方法避免了在硅胶柱色谱纯化过程中的材料损失。接种禾谷镰刀菌的水稻粗提物中的DON和3-乙酰脱氧雪腐镰刀菌烯醇被转化为三乙酰脱氧雪腐镰刀菌烯醇；乙酰化产物比DON更容易通过硅胶色谱纯化。经过水解并在活性炭-氧化铝柱上进一步纯化后，以每千克水稻高达450毫克DON的产率回收了纯度为71%的DON。随后在Sephadex LH20柱上进行分离，得到了纯度大于90%的DON。
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