Ishii K, Sawano M, Ueno Y, Tsunoda H
Appl Microbiol. 1974 Apr;27(4):625-8. doi: 10.1128/am.27.4.625-628.1974.
One hundred sixty-six isolates of Fusarium spp. from domestic cereal grains, feed, and other sources were examined for their ability to produce zearalenone on autoclaved moist rice grains. They belonged to the following species (number of producers/number tested): F. roseum (9/28), F. roseum (Culmorum) (3/4), F. roseum (Gibbosum) (2/5), F. roseum (Avenaceum) (1/2), F. roseum (Scirpi) (0/1), F. tricinctum (1/4), F. tricinctum (Sporotrichiella) (0/7), F. lateritium (1/1), F. episphaeria (0/2), F. moniliforme (0/3), F. oxysporum (0/12), F. rigidiusculum (0/4), F. solani (0/4), F. splendens (0/1), F. nivale (0/2), and Fusarium spp. (15/86). Zearalenone was isolated from molded rice by ethanol extraction and purified by column chromatography. Selected isolates of F. roseum M-3-2 and F. roseum (Gibbosum) A-O-2 produced 50 to 100 mg of zearalenone per kg of rice. Increased yields (250 to 407 mg/kg of rice) were obtained by F. roseum M-3-2 when the substrate was supplemented with 1% peptone.
对从国产谷物、饲料及其他来源分离得到的166株镰刀菌进行了检测,以考察它们在经高压灭菌的潮湿米粒上产生玉米赤霉烯酮的能力。它们属于以下菌种(产毒菌株数/检测菌株数):粉红镰刀菌(9/28)、禾谷镰刀菌(3/4)、木贼镰刀菌(2/5)、燕麦镰刀菌(1/2)、尖孢镰刀菌(0/1)、三线镰刀菌(1/4)、三线镰刀菌(孢梗束变种)(0/7)、砖红镰刀菌(1/1)、附球镰刀菌(0/2)、串珠镰刀菌(0/3)、尖镰孢菌(0/12)、硬皮镰刀菌(0/4)、茄病镰刀菌(0/4)、灿烂镰刀菌(0/1)、雪腐镰刀菌(0/2)以及镰刀菌属(15/86)。通过乙醇萃取从发霉的米粒中分离出玉米赤霉烯酮,并通过柱色谱法进行纯化。粉红镰刀菌M-3-2和木贼镰刀菌A-O-2的选定菌株在每千克大米上产生50至100毫克玉米赤霉烯酮。当底物添加1%蛋白胨时,粉红镰刀菌M-3-2的产量增加(250至407毫克/千克大米)。