Collaborative Innovation Center of Biomass Energy, Henan Agricultural University, Zhengzhou, China.
Bioengineered. 2020 Dec;11(1):291-300. doi: 10.1080/21655979.2020.1736239.
The main aim of this study was to investigate the effects of the initial pH values of the buffer on photo-fermentation biohydrogen production. Hydrogen production and the kinetics of it under different initial pH values were analyzed. Effects of initial pH values on reducing sugar consumption, hydrogen production rate, and byproduct production were evaluated at initial pH values of 5-7. The results showed that initial pH values of phosphate buffer had a significant effect on biohydrogen production via photo-fermentation. With the initial pH value of phosphate buffer at 6.0, the cumulative hydrogen production reached its maximum, 569.6 mL. The maximum hydrogen production rate was 23.96 mL/h at the initial pH value of 6.5. With the initial pH values at 5.0 and 7.5, the maximum hydrogen production rates were becoming lower, only 5.59 mL/h and 5.42 mL/h, respectively. And with the increase in pH values, the peak period of hydrogen production was gradually delayed, indicating that the alkaline environment had a negative effect on the ability of photosynthetic bacteria. This study revealed the influence of phosphate buffer initial pH values on the biohydrogen production via photo-fermentation and aimed to provide a scientific reference for further improving the theory and technology for biohydrogen production from biomass.
本研究的主要目的是探讨缓冲初始 pH 值对光发酵产氢的影响。分析了不同初始 pH 值下的产氢和动力学。在初始 pH 值为 5-7 时，评估了初始 pH 值对还原糖消耗、产氢率和副产物生成的影响。结果表明，缓冲初始 pH 值对光发酵产氢有显著影响。在磷酸盐缓冲初始 pH 值为 6.0 时，累积产氢量达到最大值，为 569.6 mL。初始 pH 值为 6.5 时，最大产氢率为 23.96 mL/h。在初始 pH 值为 5.0 和 7.5 时，最大产氢率降低，分别为 5.59 mL/h 和 5.42 mL/h。随着 pH 值的增加，产氢的高峰期逐渐延迟，表明碱性环境对光合细菌的产氢能力有负面影响。本研究揭示了磷酸盐缓冲初始 pH 值对光发酵产氢的影响，旨在为进一步提高生物质产氢的理论和技术提供科学参考。
