Filtenborg O, Frisvad J C, Svendsen J A
Appl Environ Microbiol. 1983 Feb;45(2):581-5. doi: 10.1128/aem.45.2.581-585.1983.
A simple screening method for molds producing the intracellular mycotoxins brevianamide A, citreoviridin, cyclopiazonic acid, luteoskyrin, penitrem A, roquefortine C, sterigmatocystin, verruculogen, viomellein, and xanthomegnin was developed. After removing an agar plug from the mold culture, the mycelium on the plug is wetted with a drop of methanol-chloroform (1:2). By this treatment the intracellular mycotoxins are extracted within seconds and transferred directly to a thin-layer chromatography plate by immediately placing the plug on the plate while the mycelium is still wet. After removal of the plug, known thin-layer chromatographic procedures are carried out. The substrate (Czapek yeast autolysate agar) and growth conditions (25 degrees C for 7 days) used by Penicillium taxonomists proved suitable for the production of the mycotoxins investigated when 60 known toxigenic isolates and 865 cultures isolated from foods and feedstuffs were tested with this screening method.
开发了一种用于筛选产生细胞内霉菌毒素短柄酰胺A、黄绿青霉素、环匹阿尼酸、藤黄绿菌素、青霉震颤素A、罗克福特菌素C、杂色曲霉素、疣孢菌素、黄绿青霉素和黄天精的霉菌的简单筛选方法。从霉菌培养物中取出一个琼脂塞后，用一滴甲醇 - 氯仿（1:2）润湿塞子上的菌丝体。通过这种处理，细胞内霉菌毒素在几秒钟内被提取出来，并在菌丝体仍湿润时立即将塞子放在薄层色谱板上，直接转移到薄层色谱板上。取出塞子后，进行已知的薄层色谱程序。当用这种筛选方法测试60种已知产毒分离株以及从食品和饲料中分离出的865种培养物时，青霉分类学家使用的底物（察氏酵母自溶物琼脂）和生长条件（25℃培养7天）被证明适合用于所研究霉菌毒素的产生。
