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野生大豆 WRKY20 在拟南芥中的表达增强了耐旱性并调节了 ABA 信号通路。

Expression of wild soybean WRKY20 in Arabidopsis enhances drought tolerance and regulates ABA signalling.

机构信息

Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin 150030, China.

出版信息

J Exp Bot. 2013 May;64(8):2155-69. doi: 10.1093/jxb/ert073. Epub 2013 Apr 20.

Abstract

The WRKY-type transcription factors are involved in plant development and stress responses, but how the regulation of stress tolerance is related to plant development is largely unknown. GsWRKY20 was initially identified as a stress response gene using large-scale Glycine soja microarrays. Quantitative reverse transcription-PCR (qRT-PCR) showed that the expression of this gene was induced by abscisic acid (ABA), salt, cold, and drought. Overexpression of GsWRKY20 in Arabidopsis resulted in a decreased sensitivity to ABA during seed germination and early seedling growth. However, compared with the wild type, GsWRKY20 overexpression lines were more sensitive to ABA in stomatal closure, and exhibited a greater tolerance to drought stress, a decreased water loss rate, and a decreased stomatal density. Moreover, microarray and qRT-PCR assays showed that GsWRKY20 mediated ABA signalling by promoting the expression of negative regulators of ABA signalling, such as AtWRKY40, ABI1, and ABI2, while repressing the expression of the positive regulators of ABA, for example ABI5, ABI4, and ABF4. Interestingly, GsWRKY20 also positively regulates the expression of a group of wax biosynthetic genes. Further, evidence is provided to support that GsWRKY20 overexpression lines have more epicuticular wax crystals and a much thicker cuticle, which contribute to less chlorophyll leaching compared with the wild type. Taken together, the findings reveal an important role for GsWRKY20 in enhancing drought tolerance and regulating ABA signalling.

摘要

WRKY 型转录因子参与植物发育和应激反应,但应激耐受性的调节与植物发育之间的关系在很大程度上尚不清楚。GsWRKY20 最初是使用大豆大规模微阵列鉴定为应激反应基因。定量反转录-PCR(qRT-PCR)显示,该基因的表达受脱落酸(ABA)、盐、冷和干旱诱导。GsWRKY20 在拟南芥中的过表达导致在种子萌发和早期幼苗生长过程中对 ABA 的敏感性降低。然而,与野生型相比,GsWRKY20 过表达系在气孔关闭中对 ABA 更敏感,并且对干旱胁迫具有更高的耐受性、降低的水分损失率和降低的气孔密度。此外,微阵列和 qRT-PCR 分析表明,GsWRKY20 通过促进 ABA 信号转导的负调节剂的表达来介导 ABA 信号转导,例如 AtWRKY40、ABI1 和 ABI2,同时抑制 ABA 的正调节剂的表达,例如 ABI5、ABI4 和 ABF4。有趣的是,GsWRKY20 还正向调节一组蜡生物合成基因的表达。此外,提供的证据表明 GsWRKY20 过表达系具有更多的表皮蜡晶体和更厚的角质层,与野生型相比,这有助于减少叶绿素浸出。总之,这些发现揭示了 GsWRKY20 在增强耐旱性和调节 ABA 信号转导中的重要作用。

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