Research and Development Center, Nippon Meat Packers, Inc., Tsukuba, Japan.
Anim Sci J. 2013 May;84(5):434-441. doi: 10.1111/asj.12020. Epub 2013 Jan 9.
From porcine rectal swabs or feces, we isolated lactic acid bacteria and used porcine Peyer's patch cells to select them for inducibility of IgA production as an indicator of probiotic effects. The strain selected as a new probiotic was named 'Enterococcus faecium NHRD IHARA'. To verify the probiotic effects of this strain in vivo, 536 piglets at age 25 days were assigned to either the trial group, which administrated the strain, or the control group. An increase in IgA in the feces was observed at age 45 days (P < 0.05 compared with the control group); a significant increase in serum IgA was also observed at the end of the study (P < 0.01) in the trial group. In addition, significant differences between the groups in terms of body weight (P < 0.05) and average daily gain (P < 0.01) were observed. The rate of detection of swine-pathogenic Escherichia coli gene in the feces tended to be lower in the trial group than in the controls. The novel probiotic strain; E. faecium NHRD IHARA may have beneficial effects on swine growth by inducing IgA production and reducing rates of colonization by pathogens in the body.
从猪直肠拭子或粪便中，我们分离出乳酸菌，并使用猪派伊尔斑细胞选择它们，以诱导 IgA 产生作为益生菌作用的指标。被选为新型益生菌的菌株被命名为“屎肠球菌 NHRD IHARA”。为了验证该菌株在体内的益生菌作用，将 25 日龄的 536 头仔猪分为试验组和对照组，分别给予该菌株和对照组。在 45 日龄时观察到粪便中 IgA 增加（与对照组相比 P<0.05）；在研究结束时（与对照组相比 P<0.01），试验组血清 IgA 也显著增加。此外，试验组和对照组之间在体重（P<0.05）和平均日增重（P<0.01）方面也存在显著差异。粪便中猪源致病性大肠杆菌基因的检出率在试验组中低于对照组。新型益生菌菌株屎肠球菌 NHRD IHARA 可能通过诱导 IgA 产生和降低病原体在体内定植率，对猪的生长产生有益影响。
