Sukegawa Shin, Ihara Yasuhiro, Yuge Kaoruko, Rao Shengbin, Oka Kentaro, Arakawa Fumihiro, Fujimura Tatsuya, Murakami Hiroshi, Kurazono Hisao, Takahashi Motomichi, Morimatsu Fumiki
Research and Development Center, Nippon Meat Packers, Inc., Tsukuba, Japan.
Anim Sci J. 2014 Apr;85(4):454-60. doi: 10.1111/asj.12163. Epub 2014 Jan 23.
Probiotic bacteria such as lactic acid bacteria (LAB) have recently received attention as candidates for alternative anti-microbial feed additives. We previously isolated Enterococcus faecium strain NHRD IHARA (FERM BP-11090, NHRD IHARA strain) and reported its probiotic efficacy. However, we have not determined the effect of oral administration of heat-killed cells of this strain. Here, we performed two experiments to investigate the effect of oral administration of the heat-killed NHRD IHARA strain on post-weaning piglets. In Experiment 1, there was a significant improvement in growth performance (P = 0.04) and increase in serum immunoglobulin A (IgA) production (P = 0.03) in the group fed heat-killed cells. These results were similar to previous results we obtained with live cells. We also found changes in serum and fecal IgA production that were unrelated to the patterns of microbiotal change. In Experiment 2, we detected a significant improvement in villus growth in the jejunum (P = 0.0002). In conclusion, oral administration of the heat-killed NHRD IHARA strain in post-weaning piglets had the same efficacy as administration of the live strain. The heat-killed NHRD IHARA strain can be used as feed additives to improve pig growth and health on commercial farms.
诸如乳酸菌（LAB）等益生菌最近作为替代抗菌饲料添加剂的候选物受到关注。我们之前分离出了屎肠球菌NHRD IHARA菌株（FERM BP - 11090，NHRD IHARA菌株）并报道了其益生菌功效。然而，我们尚未确定口服该菌株热灭活细胞的效果。在此，我们进行了两项实验来研究口服热灭活的NHRD IHARA菌株对断奶仔猪的影响。在实验1中，喂食热灭活细胞的组在生长性能方面有显著改善（P = 0.04），血清免疫球蛋白A（IgA）产量增加（P = 0.03）。这些结果与我们之前用活细胞获得的结果相似。我们还发现血清和粪便中IgA产量的变化与微生物群落变化模式无关。在实验2中，我们检测到空肠绒毛生长有显著改善（P = 0.0002）。总之，在断奶仔猪中口服热灭活的NHRD IHARA菌株与口服活菌株具有相同的功效。热灭活的NHRD IHARA菌株可用作饲料添加剂，以改善商业农场中猪的生长和健康状况。
