Department of Medical Microbiology & Immunology and Li Ka Shing Institute of Virology, 6020H Katz Group Centre, University of Alberta, Edmonton, AB Canada T6G 2H7.
Virology. 2013 Jul 20;442(1):59-66. doi: 10.1016/j.virol.2013.03.025. Epub 2013 Apr 19.
Vaccinia virus (VACV) strain TianTan was used for much of China's modern history to vaccinate against smallpox, however the only genome sequence contains errors. We have sequenced additional examples of TianTan to obtain a better picture of this important virus. We detected two different subclones. One (TP03) encodes large deletions in the terminal repeats that extend into both VEGF genes and create a small plaque variant. The second clone (TP05) encodes a nearly intact complement of genes in the terminal repeats, except for an insertion of sequences resembling the telomeric 69 bp repeats. The TP05 genome spans 196,260 bp and encodes 219 genes. The revised sequence documents the integrity of all the genes in the conserved virus core. Phylogenetic methods show that TianTan belongs to a unique clade of VACV, but probably also share a common origin with strains belonging to the Copenhagen/Lister lineage and distinct from the Wyeth/Dryvax lineage.
痘苗病毒（VACV）菌株天坛曾被用于中国近代历史上的大部分时间来预防天花，但唯一的基因组序列存在错误。我们已经对更多的天坛病毒进行了测序，以更好地了解这种重要的病毒。我们检测到了两种不同的亚克隆。一个（TP03）在末端重复序列中编码了大的缺失，这些缺失延伸到两个 VEGF 基因，并产生了一个小斑块变体。第二个克隆（TP05）编码了末端重复序列中几乎完整的基因，除了插入了类似于端粒 69bp 重复的序列。TP05 基因组跨度为 196260bp，编码 219 个基因。修订后的序列记录了所有保守病毒核心基因的完整性。系统发育方法表明，天坛病毒属于独特的痘苗病毒分支，但可能也与属于哥本哈根/李斯特谱系的菌株有共同的起源，与惠氏/干燥疫苗谱系不同。
