Guirakhoo F, Heinz F X, Dippe H, Kunz C
Institut für Virologie, Universität, Wien.
Zentralbl Bakteriol. 1990 Apr;272(4):477-84. doi: 10.1016/s0934-8840(11)80049-6.
Human sera obtained after tick-borne encephalitis (TBE) without prior vaccination were compared with sera from patients after a vaccination breakdown. Most sera previously shown to have high titers of IgG and IgM against TBE virus as detected in the ELISA and hemagglutination inhibition (HI) tests also reacted in Western blot with TBE virus E protein which is involved in virus neutralization. The serum of a patient with a vaccination breakdown, however, reacted only very weakly with the E protein in the Western blot in spite of a high amount of antibodies detectable in ELISA. Using SDS-denaturated virus as an antigen in ELISA (imitating the blotting condition), this serum revealed a significant reduction in its reactivity with denatured virus compared to the control sera. This indicates that the patient had an insufficient immune response against certain denaturation resistant epitopes which might contribute to development of disease despite vaccination. The analysis of the immune response of human sera at the epitope level revealed a characteristic "fingerprint" for each serum reflecting the genetic control of the production of antibody populations against different antigenic determinants.
将未接种疫苗而感染蜱传脑炎（TBE）后的人血清与疫苗接种失败患者的血清进行比较。在酶联免疫吸附测定（ELISA）和血凝抑制（HI）试验中，大多数先前显示对TBE病毒具有高滴度IgG和IgM的血清，在免疫印迹中也与参与病毒中和的TBE病毒E蛋白发生反应。然而，一名疫苗接种失败患者的血清，尽管在ELISA中可检测到大量抗体，但在免疫印迹中与E蛋白的反应却非常微弱。在ELISA中使用SDS变性病毒作为抗原（模拟印迹条件），与对照血清相比，该血清与变性病毒的反应性显著降低。这表明该患者对某些抗变性表位的免疫反应不足，这可能是尽管接种了疫苗仍导致疾病发生的原因。在表位水平对人血清免疫反应的分析揭示了每种血清的特征性“指纹”，反映了针对不同抗原决定簇的抗体群体产生的遗传控制。
