Department of Food Science & Technology, Ramin Agricultural & Natural Resources University, Ahvaz, Iran.
Int J Biol Macromol. 2013 Sep;60:427-36. doi: 10.1016/j.ijbiomac.2013.04.050. Epub 2013 Apr 20.
The effects of extraction temperature, extraction time, the ratio of water to raw material, and number of extraction on extraction yield of crude polysaccharides from the leaves of Malva sylvestris (MSLCP) were optimized by statistical analysis using response surface methodology. The response surface methodology (RSM) was used to optimize MSLCP extraction yield by implementing the CCD design. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods (ANOVA). Statistical analysis of the results showed that the linear and quadratic terms of these four variables had significant effects. The optimal conditions for higher extraction yield of MSLCP were extraction temperature: 90 °C, extraction time: 4 h, number of extraction: 2 and the ratio of water to raw material: 21. Under these conditions, the experimental yield was 8.377±0.38%, which is well in close agreement with the value predicted by the model 8.608%. The results demonstrated that MSLCP had strong scavenging activities in vitro on DPPH and hydroxyl radicals. Overall, MSLCP may have potential applications in the medical and food industries.
采用响应面法中的中心组合设计（CCD）对水提温度、提取时间、料液比和提取次数对野芝麻叶粗多糖（MSLCP）提取率的影响进行了优化。利用多元回归分析对实验数据进行拟合，并采用适当的统计方法（方差分析）对其进行分析。结果表明，这四个因素的线性项和二次项对提取率均有显著影响。提取 MSLCP 的最佳条件为：提取温度 90℃、提取时间 4 h、提取次数 2 次、料液比 21。在此条件下，实验提取率为 8.377±0.38%，与模型预测值 8.608%吻合较好。结果表明，MSLCP 对 DPPH 和羟基自由基具有较强的体外清除能力。总之，MSLCP 在医药和食品工业中可能具有潜在的应用价值。
