Alkahtani Jawaher, Soliman Elshikh Mohamed, Almaary Khalid S, Ali Shafat, Imtiyaz Zuha, Bilal Ahmad Sheikh
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Biochemistry, Government Medical College (GMC-Srinagar), 190010 India.
Saudi J Biol Sci. 2020 Nov;27(11):2929-2935. doi: 10.1016/j.sjbs.2020.08.014. Epub 2020 Aug 13.
Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities. The present investigation aimed to examine the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides. The antibacterial effects and as well as of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability. Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method. Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines. The findings showed that GC polysaccharides MIC were 1.06 and 0.56 mg mL against E. coli and S. aureus, respectively. Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols. The IC values of all tested parameters were measured against standard ascorbic acid antioxidant agent. The GC polysaccharides diminish the cell viability percentage of HeLa and HepG2 in a concentration dependent manner. GC polysaccharides at a dose of 500 µg ml exhibited higher anti-tumor activity in both HeLa (65.33 ± 3.75%) and HepG2 (60.33 ± 3.48%). The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
植物多糖是具有不同药理和生物活性的药物复合物的无尽来源。本研究旨在检测水田芥（GC）多糖的抗菌、抗氧化和细胞毒性潜力。通过琼脂扩散试验、最低抑菌浓度（MIC）、外细胞膜和内细胞膜通透性检测GC多糖的抗菌效果。通过自由基DPPH清除、超氧阴离子清除、羟基自由基清除、还原能力检测和过氧化氢法检测GC多糖的抗氧化潜力。通过MTT试验评估GC多糖在人宫颈癌细胞系（HeLa）和肝癌细胞系（HepG2）中的细胞毒性潜力。结果表明，GC多糖对大肠杆菌和金黄色葡萄球菌的MIC分别为1.06和0.56mg/mL。与标准抑制剂相比，GC多糖以非常剂量依赖的方式显示出基本的抑制作用，并且清除活性氧（ROS）的显著作用也归因于大量的亲水性多酚。所有测试参数的IC值均以标准抗坏血酸抗氧化剂为对照进行测量。GC多糖以浓度依赖的方式降低HeLa和HepG2的细胞活力百分比。500μg/ml剂量的GC多糖在HeLa（65.33±3.75%）和HepG2（60.33±3.48%）中均表现出较高的抗肿瘤活性。本研究获得的结果表明，GC多糖具有抗菌作用，可能是天然抗氧化剂的来源，并且对不同癌细胞系具有细胞毒性作用。
