Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University, Mollasani, Ahvaz, Iran.
Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University, Mollasani, Ahvaz, Iran.
Carbohydr Polym. 2014 Nov 4;112:396-403. doi: 10.1016/j.carbpol.2014.06.024. Epub 2014 Jun 19.
Box-Behnken design was employed to optimize the extraction conditions for polysaccharides from the leaves of motherwort (Leonurus cardiaca L.). Three independent variables including extraction temperature (60-100 °C), extraction time (60-120 min), and the ratio of water to raw material (20-60) were investigated. The results revealed that the quadratic and linear terms of three factors had strong effects on the extraction yield of polysaccharides from motherwort leaf. The best extraction conditions for the yield of polysaccharide (LCLP) was extraction temperature of 81.4 °C, time of 106.6 min and the ratio of water to raw material of 45.2. Under the optimal conditions, the extraction yield of LCLP was 9.17 ± 0.39%, which was well matched with value predicted by the model 9.26%. The results indicated that the purified LCLP exerted obvious scavenging effects on free radicals in vitro. Furthermore, motherwort polysaccharides could be used as a novel antimicrobial additive.
采用 Box-Behnken 设计对益母草（Leonurus cardiaca L.）叶多糖的提取条件进行优化。考察了 3 个独立变量，包括提取温度（60-100°C）、提取时间（60-120 分钟）和料液比（20-60）。结果表明，3 个因素的二次项和线性项对益母草叶多糖提取率有较强的影响。多糖（LCLP）得率最佳的提取条件为：提取温度 81.4°C，时间 106.6 分钟，料液比 45.2。在最优条件下，LCLP 的提取率为 9.17±0.39%，与模型预测值 9.26%吻合较好。结果表明，纯化后的 LCLP 对自由基具有明显的体外清除作用。此外，益母草多糖可用作新型抗菌添加剂。
