Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
J Ethnopharmacol. 2013 Jun 21;148(1):271-6. doi: 10.1016/j.jep.2013.04.025. Epub 2013 Apr 21.
The root of Scutellaria baicalensis Georgi, also called Huangqin in China, is an herbal-based nutraceutical which is usually used in Chinese medicated diet (CMD). As an abundant ingredient in Huangqin, wogonoside is a flavonoid glycoside. The present work investigated the anti-inflammatory activities of wogonoside in lipopolysaccharides (LPS)-induced RAW264.7 cells.
RAW264.7 cells were used. The inhibition of wogonoside against nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in LPS-induced RAW264.7 cells were measured. Additionally, the effects of wogonoside on mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX2), TNF-α and IL-6 were also investigated.
Wogonoside not only dose-dependently decreased the production of inflammatory mediators including NO and PGE2 but also inhibited the release of pro-inflammatory cytokines including TNF-α and IL-6 in LPS-induced RAW264.7 cells. Furthermore, wogonoside possessed significantly in vitro inhibitory effects on the gene expression of iNOS, COX2, TNF-α and IL-6.
These results suggest that wogonoside may be used as a functional food component for prevention and treatment of inflammation.
黄芩的根，在中国也被称为黄芩，是一种基于草药的营养保健品，通常用于中药饮食（CMD）。作为黄芩中的一种丰富成分，黄芩苷是一种黄酮类糖苷。本研究探讨了黄芩苷在脂多糖（LPS）诱导的 RAW264.7 细胞中的抗炎活性。
使用 RAW264.7 细胞。测量黄芩苷对 LPS 诱导的 RAW264.7 细胞中一氧化氮（NO）、前列腺素 E2（PGE2）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的抑制作用。此外，还研究了黄芩苷对诱导型一氧化氮合酶（iNOS）、环氧化酶 2（COX2）、TNF-α和 IL-6 基因表达的影响。
黄芩苷不仅剂量依赖性地降低了包括 NO 和 PGE2 在内的炎症介质的产生，还抑制了 LPS 诱导的 RAW264.7 细胞中促炎细胞因子 TNF-α和 IL-6 的释放。此外，黄芩苷对 iNOS、COX2、TNF-α和 IL-6 的基因表达具有显著的体外抑制作用。
这些结果表明，黄芩苷可用作预防和治疗炎症的功能性食品成分。
