Departamento de Física Aplicada, Universidade de Vigo, 36310, Vigo, Spain.
Nanoscale. 2013 Jun 7;5(11):4797-807. doi: 10.1039/c3nr34358h. Epub 2013 Apr 24.
The polyelectrolyte-DNA complexation method to form magnetoplexes using silica-coated iron oxide magnetic nanoparticles as inorganic substrates is an attractive and promising process in view of the potential applications including magnetofection, DNA extraction and purification, and directed assembly of nanostructures. Herein, we present a systematic physico-chemical study that provides clear evidence of the type of interactions established, reflects the importance of the DNA length, the nanoparticle size and the ionic strength, and permits the identification of the parameters controlling both the stability and the type of magnetoplexes formed. This information can be used to develop targeted systems with properties optimized for the various proposed applications of magnetoplexes.
采用聚电解质-DNA 复合方法，以二氧化硅包覆的氧化铁磁性纳米粒子作为无机基质形成磁复合颗粒，是一种很有吸引力和前景的方法，潜在应用包括磁转染、DNA 提取和纯化以及纳米结构的定向组装。在此，我们进行了系统的物理化学研究，提供了明确的相互作用类型的证据，反映了 DNA 长度、纳米颗粒尺寸和离子强度的重要性，并确定了控制磁复合颗粒稳定性和类型的参数。这些信息可用于开发针对各种磁复合颗粒应用的靶向系统，优化其性能。
