Liu Lingling, Guo Zilong, Huang Zhenzhen, Zhuang Jiaqi, Yang Wensheng
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.
Sci Rep. 2016 Feb 25;6:22029. doi: 10.1038/srep22029.
In this work, a facile and efficient approach has been demonstrated for size-selective separation of DNA fragments by using lysine-functionalized silica particles. At a given pH, the environmental ionic strength can be utilized to alter the electrostatic interactions of lysine-functionalized silica particles with DNA fragments and in turn the DNA fragments on the silica particle surfaces, which exhibits a clear dependence on the DNA fragment sizes. By carefully adjusting the environmental pH and salt concentration, therefore, the use of the lysine-functionalized silica particles allows effective separation of binary and ternary DNA mixtures, for example, two different DNA fragments with sizes of 101 and 1073 bp, 101 and 745 bp, 101 and 408 bp, respectively, and three different DNA fragments with sizes of 101, 408 and 1073 bp.
在这项工作中，已经展示了一种通过使用赖氨酸功能化的二氧化硅颗粒对DNA片段进行尺寸选择性分离的简便有效方法。在给定的pH值下，环境离子强度可用于改变赖氨酸功能化的二氧化硅颗粒与DNA片段之间的静电相互作用，进而改变二氧化硅颗粒表面的DNA片段，这对DNA片段大小有明显的依赖性。因此，通过仔细调节环境pH值和盐浓度，使用赖氨酸功能化的二氧化硅颗粒可以有效地分离二元和三元DNA混合物，例如，分别为大小为101和1073 bp、101和745 bp、101和408 bp的两种不同DNA片段，以及大小为101、408和1073 bp的三种不同DNA片段。
