Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Biotechnol Bioeng. 2013 Oct;110(10):2795-801. doi: 10.1002/bit.24935. Epub 2013 Apr 30.
Engineering of algal cells by delivering macromolecules through cell wall and plasma membrane presents many difficulties with the conventional methods. Recent research has shown that a new delivery method, namely cell penetrating peptide (CPP), has the ability to translocate into animal, plant, fungal, and bacterial cells. This study reports the apparent translocation of CPPs into algal cells of Chlamydomonas reinhardtii and the successful delivery of the conjugated fluorochrome. Although translocation efficiency was specific to each CPP studied, pVEC (peptide vascular endothelial cadherin) showed the highest translocation efficiency in comparison with penetratin (PEN), trans-activating transcriptional (TAT) peptide, and transportan (TRA). The maximum translocation of pVEC into the algal cell was reached in 15 min of incubation at 25°C. More importantly, translocation with pVEC demonstrated an absence of cytotoxicity. Thus, we suggested that pVEC is an attractive candidate for delivering macromolecules into algal cells for use in industrial applications.
通过细胞壁和质膜输送大分子物质来工程化藻类细胞，这给传统方法带来了许多困难。最近的研究表明，一种新的输送方法，即细胞穿透肽（CPP），能够将物质转位进入动物、植物、真菌和细菌细胞。本研究报告了 CPP 明显转位进入莱茵衣藻（Chlamydomonas reinhardtii）藻类细胞，并成功传递了共轭荧光染料。尽管每种研究的 CPP 都具有特定的转位效率，但与 penetratin (PEN)、trans-activating transcriptional (TAT) 肽和 transportan (TRA) 相比，pVEC（肽血管内皮钙黏蛋白）显示出最高的转位效率。在 25°C 孵育 15 分钟时，pVEC 最大程度地转位进入藻类细胞。更重要的是，pVEC 的转位没有显示出细胞毒性。因此，我们认为 pVEC 是将大分子物质递送入藻类细胞用于工业应用的有吸引力的候选物。
