鼻咽癌中所选核糖体蛋白基因的表达趋势
Expression trend of selected ribosomal protein genes in nasopharyngeal carcinoma.
作者信息
Ma Xiang-Ru, Sim Edmund Ui-Hang, Ling Teck-Yee, Tiong Thung-Sing, Subramaniam Selva Kumar, Khoo Alan Soo-Beng
机构信息
Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
出版信息
Malays J Med Sci. 2012 Oct;19(4):23-30.
BACKGROUND
Ribosomal proteins are traditionally associated with protein biosynthesis until recent studies that implicated their extraribosomal functions in human diseases and cancers. Our previous studies using GeneFishing™ DEG method and microarray revealed underexpression of three ribosomal protein genes, RPS26, RPS27, and RPL32 in cancer of the nasopharynx. Herein, we investigated the expression pattern and nucleotide sequence integrity of these genes in nasopharyngeal carcinoma to further delineate their involvement in tumourigenesis. The relationship of expression level with clinicopathologic factors was also statistically studied.
METHODS
Quantitative Polymerase Chain Reaction was performed on nasopharyngeal carcinoma and their paired normal tissues. Expression and sequence of these three genes were analysed.
RESULTS
All three ribosomal protein genes showed no significant difference in transcript expressions and no association could be established with clinicopathologic factors studied. No nucleotide aberrancy was detected in the coding regions of these genes.
CONCLUSION
There is no early evidence to substantiate possible involvement of RPS26, RPS27, and RPL32 genes in NPC tumourigenesis.
背景
核糖体蛋白传统上与蛋白质生物合成相关，直到最近的研究表明它们在人类疾病和癌症中具有核糖体以外的功能。我们之前使用基因钓鱼™差异表达基因方法和微阵列进行的研究显示，三种核糖体蛋白基因RPS26、RPS27和RPL32在鼻咽癌中表达不足。在此，我们研究了这些基因在鼻咽癌中的表达模式和核苷酸序列完整性，以进一步阐明它们在肿瘤发生中的作用。还对表达水平与临床病理因素的关系进行了统计学研究。
方法
对鼻咽癌及其配对的正常组织进行定量聚合酶链反应。分析这三个基因的表达和序列。
结果
所有三个核糖体蛋白基因在转录表达上均无显著差异，且与所研究的临床病理因素无关联。在这些基因的编码区未检测到核苷酸异常。
结论
没有早期证据证实RPS26、RPS27和RPL32基因可能参与鼻咽癌的肿瘤发生。
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