Sim Edmund Ui-Hang, Chan Stella Li-Li, Ng Kher-Lee, Lee Choon-Weng, Narayanan Kumaran
Department of Molecular Biology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Malaysia.
Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Dis Markers. 2016;2016:5179594. doi: 10.1155/2016/5179594. Epub 2016 Nov 28.
Apart from their canonical role in ribosome biogenesis, there is increasing evidence of ribosomal protein genes' involvement in various cancers. A previous study by us revealed significant differential expression of three ribosomal protein genes (, , and ) between cell lines derived from tumor and normal nasopharyngeal epithelium. However, the results therein were based on a semiquantitative assay, thus preliminary in nature. Herein, we provide findings of a deeper analysis of these three genes in the context to nasopharyngeal carcinoma (NPC) tumorigenesis. Their expression patterns were analyzed in a more quantitative manner at transcript level. Their protein expression levels were also investigated. We showed results that are contrary to previous report. Rather than downregulation, these genes were significantly overexpressed in NPC cell lines compared to normal control at both transcript and protein levels. Nevertheless, their association with NPC has been established. Immunoprecipitation pulldown assays indicate the plausible interaction of either RPeL27 or RPeL43 with POTEE/TUBA1A and ACTB/ACTBL2 complexes. In addition, RPeL43 is shown to bind with MRAS and EIF2S1 proteins in a NPC cell line (HK1). Our findings support , , and as potential markers of NPC and provide insights into the interaction targets of RPeL27 and RPeL43 proteins.
除了在核糖体生物合成中的经典作用外，越来越多的证据表明核糖体蛋白基因参与了各种癌症。我们之前的一项研究揭示了三种核糖体蛋白基因（、和）在源自肿瘤和正常鼻咽上皮的细胞系之间存在显著差异表达。然而，其中的结果基于半定量分析，因此本质上是初步的。在此，我们提供了在鼻咽癌（NPC）肿瘤发生背景下对这三个基因进行更深入分析的结果。在转录水平以更定量的方式分析了它们的表达模式。还研究了它们的蛋白质表达水平。我们展示的结果与之前的报告相反。与正常对照相比，这些基因在NPC细胞系的转录本和蛋白质水平上均显著过表达，而非下调。尽管如此，它们与NPC的关联已经确立。免疫沉淀下拉分析表明RPeL27或RPeL43与POTEE/TUBA1A和ACTB/ACTBL2复合物之间可能存在相互作用。此外，在一个NPC细胞系（HK1）中，RPeL43被证明与MRAS和EIF2S1蛋白结合。我们的研究结果支持、和作为NPC的潜在标志物，并为RPeL27和RPeL43蛋白的相互作用靶点提供了见解。
