Dalvi Shubhangi Mahesh, Patil Vinayak Wamanrao, Ramraje Nagsen Nirgun, Phadtare Jaising Marutrao, Gujarathi Sarita Uday
Department of Biochemistry Grant Government Medical College and Sir J.J. Group of Hospitals Byculla Mumbai 400008, India.
Malays J Med Sci. 2013 Jan;20(1):21-30.
Lipid peroxidation, nitric oxide, carbonyl protein, causing production of reactive oxygen and reactive nitrogen intermediates that lead to oxidative, nitrosative stress. The stress is found to cause deterioration in the cellular function, mutagenesis, and DNA damage. The oxidative stress is correlated with the antioxidant vitamins status.
Newly diagnosed cultured positive sputum pulmonary category I, II, III (n = 100 each), extra pulmonary category I (n = 35) before and after directly observed short course treatment of six months vitamins, by HPLC.
Oxidative parameter levels were significantly increased, and activities of vitamins were found to be significantly decreased in subjects of all categories of pulmonary and extra pulmonary tuberculosis. Positive correlations between nitric oxide, carbonyl protein, and lipid peroxidation were seen among them. Negative correlations between nitric oxide, carbonyl protein, lipid peroxidation with vitamin E, C, A were seen in tuberculosis (two sided P < 0.01).
Increase oxidative stress and nitrosative stress, leading to protein carbonyl formation in tuberculosis. The increased protein carbonyl, hampers many important functions of proteins. The changes were reversed after six months of antitubercular treatment in patients with good recovery but increase stress was not completely reversed.
脂质过氧化、一氧化氮、羰基蛋白会导致活性氧和活性氮中间体的产生，进而引发氧化应激和亚硝化应激。研究发现，这种应激会导致细胞功能恶化、诱变和DNA损伤。氧化应激与抗氧化维生素状态相关。
通过高效液相色谱法，对新诊断的痰培养阳性的肺部Ⅰ、Ⅱ、Ⅲ类（各100例）以及肺外Ⅰ类（35例）患者在接受六个月直接观察短程治疗前后的维生素情况进行检测。
在所有类型的肺结核和肺外结核患者中，氧化参数水平显著升高，而维生素活性显著降低。其中，一氧化氮、羰基蛋白和脂质过氧化之间呈正相关。在结核病患者中，一氧化氮、羰基蛋白、脂质过氧化与维生素E、C、A之间呈负相关（双侧P<0.01）。
结核病中氧化应激和亚硝化应激增加，导致蛋白质羰基形成。蛋白质羰基增加会妨碍蛋白质的许多重要功能。经过六个月抗结核治疗后，恢复良好的患者这些变化有所逆转，但增加的应激并未完全逆转。
