Department of Chemistry, University of California, Irvine, California 92697-2025, USA.
J Am Chem Soc. 2013 May 22;135(20):7761-7. doi: 10.1021/ja4028082. Epub 2013 May 13.
The sensitive detection of cancer biomarkers in urine could revolutionize cancer diagnosis and treatment. Such detectors must be inexpensive, easy to interpret, and sensitive. This report describes a bioaffinity matrix of viruses integrated into PEDOT films for electrochemical sensing of prostate-specific membrane antigen (PSMA), a prostate cancer biomarker. High sensitivity to PSMA resulted from synergistic action by two different ligands to PSMA on the same phage particle. One ligand was genetically encoded, and the secondary recognition ligand was chemically synthesized to wrap around the phage. The dual ligands result in a bidentate binder with high-copy, dense ligand display for enhanced PSMA detection through a chelate-based avidity effect. Biosensing with virus-PEDOT films provides a 100 pM limit of detection for PSMA in synthetic urine without requiring enzymatic or other amplification.
尿液中癌症生物标志物的灵敏检测可能会彻底改变癌症的诊断和治疗方式。此类检测器必须价格低廉、易于解读且灵敏。本报告介绍了一种将病毒整合到聚(3,4-乙烯二氧噻吩)薄膜中的生物亲和基质，用于电化学检测前列腺特异性膜抗原（PSMA），这是一种前列腺癌生物标志物。由于同一噬菌体颗粒上的两种不同 PSMA 配体的协同作用，该传感器对 PSMA 具有高灵敏度。一种配体是基因编码的，而二级识别配体则通过化学合成来包裹噬菌体。这种双配体形成了一种双齿结合物，具有高拷贝、高密度配体展示，通过螯合亲和力效应增强了 PSMA 的检测。使用病毒-PEDOT 薄膜进行生物传感，可在无需酶或其他放大的情况下，以 100 pM 的检测限检测合成尿液中的 PSMA。
