Department of Chemistry, Fudan University, Shanghai, 200438, China.
Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China.
Mikrochim Acta. 2021 Aug 2;188(8):283. doi: 10.1007/s00604-021-04934-7.
Tumor exosomes that inherit specific molecules from their parent cells are emerging as ideal biomarkers in cancer diagnostics. Most currently available exosome isolation and detection methods are time-consuming and non-specific; thus, rapid and specific exosome detection methods are needed both clinically and in research. Here, a dual-functional platform is reported composed of reversible conjunction and "off-on" signal responses. FeO@SiO@TiO particles with high affinity were applied to capture exosomes, and model exosomes could be isolated from solution within 20 min with a capture efficiency of 91.5%. An "on-off" fluorescence response PSMA aptasensor was constructed with improved selectivity to detect tumor exosomes by recording the fluorescence intensity with λ = 557/580 nm. The standard curve for detecting tumor exosomes with the aptasensor was calculated as y = 371.7x + 66.17, ranging from 0.05 to 1 × 10 particles/μL, with R = 0.9737, and a detection limit of 5 × 10 particles/μL in solution. This method was successfully applied to clinical samples, and the results showed better performance in distinguishing prostate cancer patients and healthy samples than the traditional nanoparticle-tracking analysis (NTA) method. This rapid and accurate detection method for prostate cancer may aid in rapid clinical diagnosis. Integrating quickly TiO-based isolation with sensitive and specific "on-off" detection of PCa exosomes.
肿瘤细胞外体从其亲本细胞中继承特定分子，正在成为癌症诊断中理想的生物标志物。目前大多数可用的外体分离和检测方法既耗时又非特异性；因此，临床和研究都需要快速和特异性的外体检测方法。在这里，报道了一种由可逆连接和“开-关”信号响应组成的双重功能平台。具有高亲和力的 FeO@SiO@TiO 颗粒被应用于捕获外体，并且可以在 20 分钟内从溶液中分离出模型外体，捕获效率为 91.5%。构建了一种具有改进选择性的“开-关”荧光响应 PSMA 适体传感器，通过记录 λ=557/580nm 的荧光强度来检测肿瘤外体。用适体传感器检测肿瘤外体的标准曲线计算为 y=371.7x+66.17，范围从 0.05 到 1×10 个颗粒/μL，R=0.9737，检测限为 5×10 个颗粒/μL 在溶液中。该方法成功应用于临床样本，结果表明，与传统的纳米颗粒跟踪分析（NTA）方法相比，该方法在区分前列腺癌患者和健康样本方面具有更好的性能。这种用于快速诊断前列腺癌的快速、准确的检测方法可能有助于快速临床诊断。快速 TiO 基分离与前列腺癌外体的灵敏和特异性“开-关”检测相结合。
