Exp Dermatol. 2013 May;22(5):364-6. doi: 10.1111/exd.12133.
Antimicrobial peptides (AMP) are key players in the skin's defense system. Previous observations suggest a site- and age-dependent expression of individual AMP. We investigated the expression and secretion patterns of four important AMP in a representative collective of healthy human skin samples. Levels of psoriasin, RNase 7 and hBD-3 expression - assessed by immunohistochemistry - varied between different body localisations. Older individuals expressed hBD-2 more frequently. No gender-related expression was observed. The in vivo secretion of psoriasin, measured in skin washing fluids using ELISA, was related to body localisation and age, whereas RNase 7 secretion showed no significant differences regarding these variables. HBD-2 and -3 secretion could not be detected. Our findings suggest the usage of control samples matching localisation and approximate age (in the case of hBD-2) for comparative immunohistochemical analysis. To avoid bias through great interindividual differences, sufficient large collectives should be used for in vivo secretion analyses.
抗菌肽 (AMP) 是皮肤防御系统的关键组成部分。先前的观察表明，个体 AMP 的表达具有位置和年龄依赖性。我们研究了四种重要 AMP 在代表性的健康人体皮肤样本中的表达和分泌模式。通过免疫组织化学评估，角蛋白丝聚集素、RNase 7 和 hBD-3 的表达水平在不同的身体部位存在差异。年龄较大的个体更频繁地表达 hBD-2。未观察到与性别相关的表达。使用 ELISA 在皮肤洗液中测量的角蛋白丝聚集素的体内分泌与身体部位和年龄有关，而 RNase 7 的分泌在这些变量方面没有显著差异。无法检测到 hBD-2 和 hBD-3 的分泌。我们的研究结果表明，对于比较免疫组织化学分析，应使用匹配位置和近似年龄的对照样本（对于 hBD-2 而言）。为了避免由于个体间差异过大而产生偏差，应使用足够大的样本集进行体内分泌分析。
