Department of Chemistry, Michigan Technological University, Houghton, Michigan 49931, United States.
ACS Appl Mater Interfaces. 2013 May 22;5(10):4107-12. doi: 10.1021/am303247s. Epub 2013 May 13.
A highly water-soluble BODIPY dye bearing electron-rich o-diaminophenyl groups at 2,6-positions was prepared as a highly sensitive and selective fluorescent probe for detection of nitric oxide (NO) in living cells. The fluorescent probe displays an extremely weak fluorescence with fluorescence quantum yield of 0.001 in 10 mM phosphate buffer (pH 7.0) in the absence of NO as two electron-rich o-diaminophenyl groups at 2,6-positions significantly quench the fluorescence of the BODIPY dye via photoinduced electron transfer mechanism. The presence of NO in cells enhances the dye fluorescence dramatically. The fluorescent probe demonstrates excellent water solubility, membrane permeability, and compatibility with living cells for sensitive detection of NO.
一种高度水溶性的 BODIPY 染料，在 2，6-位带有富电子的邻二氨基苯基基团，被制备为一种用于检测活细胞中一氧化氮（NO）的高灵敏度和选择性荧光探针。在不存在 NO 的情况下，荧光探针在 10 mM 磷酸盐缓冲液（pH 7.0）中显示出极其微弱的荧光，荧光量子产率为 0.001，因为 2，6-位的两个富电子邻二氨基苯基基团通过光诱导电子转移机制显著猝灭了 BODIPY 染料的荧光。细胞中存在的 NO 会显著增强染料的荧光。该荧光探针具有出色的水溶性、膜透过性和与活细胞的相容性，可用于灵敏检测 NO。
