School of Food and Nutritional Sciences, University College Cork, Cork, Ireland.
Am J Clin Nutr. 2013 Jun;97(6):1235-42. doi: 10.3945/ajcn.112.057182. Epub 2013 Apr 24.
The Vitamin D Standardization Program (VDSP) has developed protocols for standardizing procedures of 25-hydroxyvitamin D [25(OH)D] measurement in National Health/Nutrition Surveys to promote 25(OH)D measurements that are accurate and comparable over time, location, and laboratory procedure to improve public health practice.
We applied VDSP protocols to existing ELISA-derived serum 25(OH)D data from the Irish National Adult Nutrition Survey (NANS) as a case-study survey and evaluated their effectiveness by comparison of the protocol-projected estimates with those from a reanalysis of survey serums by using liquid chromatography-tandem mass spectrometry (LC-tandem MS).
The VDSP reference system and protocols were applied to ELISA-based serum 25(OH)D data from the representative NANS sample (n = 1118). A reanalysis of 99 stored serums by using standardized LC-tandem MS and resulting regression equations yielded predicted standardized serum 25(OH)D values, which were then compared with LC-tandem MS reanalyzed values for all serums.
Year-round prevalence rates for serum 25(OH)D concentrations <30, <40, and <50 nmol/L were 6.5%, 21.9%, and 40.0%, respectively, via original ELISA measurements and 11.4%, 25.3%, and 43.7%, respectively, when VDSP protocols were applied. Differences in estimates at <30- and <40-nmol/L thresholds, but not at the <50-nmol/L threshold, were significant (P < 0.05). A reanalysis of all serums by using LC-tandem MS confirmed prevalence estimates as 11.2%, 27.2%, and 45.0%, respectively. Prevalences of serum 25(OH)D concentrations >125 nmol/L were 1.2%, 0.3%, and 0.6% by means of ELISA, VDSP protocols, and LC-tandem MS, respectively.
VDSP protocols hold a major potential for national nutrition and health surveys in terms of the standardization of serum 25(OH)D data.
维生素 D 标准化计划（VDSP）已经制定了规范国家健康/营养调查中 25-羟维生素 D [25(OH)D]测量程序的方案，以促进 25(OH)D 测量在时间、地点和实验室程序方面的准确性和可比性，从而改善公共卫生实践。
我们将 VDSP 方案应用于爱尔兰国家成人营养调查（NANS）中现有的酶联免疫吸附测定（ELISA）衍生血清 25(OH)D 数据，作为案例研究调查，并通过比较方案预测的估计值与使用液相色谱-串联质谱法（LC-串联 MS）重新分析调查血清的估计值来评估其有效性。
VDSP 参考系统和方案应用于具有代表性的 NANS 样本（n=1118）的基于 ELISA 的血清 25(OH)D 数据。使用标准化 LC-串联 MS 对 99 份储存血清进行重新分析，并得出预测标准化血清 25(OH)D 值，然后将其与所有血清的 LC-串联 MS 重新分析值进行比较。
通过原始 ELISA 测量，血清 25(OH)D 浓度<30、<40 和<50 nmol/L 的全年患病率分别为 6.5%、21.9%和 40.0%，而应用 VDSP 方案后分别为 11.4%、25.3%和 43.7%。在<30-和<40-nmol/L 阈值处的估计值差异，但在<50-nmol/L 阈值处没有差异，具有统计学意义（P<0.05）。使用 LC-串联 MS 对所有血清进行重新分析，分别证实患病率估计值为 11.2%、27.2%和 45.0%。通过 ELISA、VDSP 方案和 LC-串联 MS 分别为 1.2%、0.3%和 0.6%。
VDSP 方案在血清 25(OH)D 数据标准化方面为国家营养和健康调查提供了巨大的潜力。
