Kim Hye Kyong, Choi Young Hae, Verpoorte Robert
Natural Product Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands.
Methods Mol Biol. 2013;1011:267-75. doi: 10.1007/978-1-62703-414-2_21.
Metabolomics based on nuclear magnetic resonance (NMR) can be used to monitor the metabolite response of plants to jasmonic acid. Metabolomics experiments consist of three important steps: sample preparation, NMR analysis, and data mining. In sample preparation, a very critical factor is the selection of a proper solvent for plant material extraction because the presence of metabolites will vary according to the solvent used. For NMR analysis, an intermediate-to-polar solvent, such as a mixture of methanol and water, is a good choice. In general, (1)H-NMR spectroscopy is the standard method for metabolite profiling, whereas two-dimensional spectroscopy provides more detailed information on the metabolites. Finally, various chemometric methods can be used for data mining. Here, we describe all three steps of metabolomic analysis by means of NMR spectroscopy.
基于核磁共振（NMR）的代谢组学可用于监测植物对茉莉酸的代谢物响应。代谢组学实验包括三个重要步骤：样品制备、NMR分析和数据挖掘。在样品制备中，一个非常关键的因素是选择合适的溶剂用于植物材料提取，因为代谢物的存在会根据所用溶剂而有所不同。对于NMR分析，中等极性的溶剂，如甲醇和水的混合物，是一个不错的选择。一般来说，氢核磁共振波谱法（¹H-NMR）是代谢物谱分析的标准方法，而二维波谱法能提供有关代谢物更详细的信息。最后，各种化学计量学方法可用于数据挖掘。在此，我们通过核磁共振波谱法描述代谢组学分析的所有三个步骤。
