Flores-Sanchez Isvett Josefina, Pec Jaroslav, Fei Junni, Choi Young Hae, Dusek Jaroslav, Verpoorte Robert
Pharmacognosy Department/Metabolomics, Institute of Biology, Gorlaeus Laboratories, P.O. Box 9502, Leiden University, 2300 RA Leiden, The Netherlands.
J Biotechnol. 2009 Aug 20;143(2):157-68. doi: 10.1016/j.jbiotec.2009.05.006. Epub 2009 Jun 11.
Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with biotic and abiotic elicitors to evaluate their effect on secondary metabolism. Metabolic profiles analysed by (1)H NMR spectroscopy and principal component analysis (PCA) showed variations in some of the metabolite pools. However, no cannabinoids were found in either control or elicited cannabis cell cultures. Tetrahydrocannabinolic acid (THCA) synthase gene expression was monitored during a time course. Results suggest that other components in the signaling pathway can be controlling the cannabinoid pathway.
大麻植株会产生各种各样的次生代谢产物。用生物和非生物诱导剂处理大麻细胞培养物，以评估它们对次生代谢的影响。通过核磁共振氢谱（¹H NMR）光谱和主成分分析（PCA）分析的代谢谱显示，一些代谢物库存在变化。然而，在对照或诱导的大麻细胞培养物中均未发现大麻素。在一个时间进程中监测了四氢大麻酚酸（THCA）合酶基因的表达。结果表明，信号通路中的其他成分可能在控制大麻素途径。
