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Transferrin polymorphism detected in human urine using isoelectric focusing followed by immunoblotting.

作者信息

Kishi K, Ikehara Y, Yasuda T, Mizuta K, Sato W

机构信息

Department of Legal Medicine, Fukui Medical School, Japan.

出版信息

Forensic Sci Int. 1990 Apr;45(3):225-30. doi: 10.1016/0379-0738(90)90178-2.

DOI:10.1016/0379-0738(90)90178-2
PMID:2361645
Abstract

Genetic polymorphism of transferrin (TF) was revealed in human urine by isoelectric focusing and immunoblotting on thin-layer polyacrylamide gels. Using this technique more than 300 urine samples were examined, and correct TF typing from a small volume of urine (approx. 0.5 ml) was achieved, in comparison with the results of direct grouping for plasma. Three common phenotypes, TF C1, C2-1 and C2, were differentiated. In addition, the rare types TF C1D, C2D, and C1B were observed. The frequencies of the TF alleles in our samples were found to be: TFC1 = 0.7265, TFC2 = 0.2624, TFD = 0.0083 and TFB = 0.0028.

摘要

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