School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China.
Carbohydr Polym. 2013 Jun 5;95(1):114-22. doi: 10.1016/j.carbpol.2013.03.015. Epub 2013 Mar 13.
In the present study, the extraction, purification, characterization and biological activities of polysaccharides from the seeds of Amomum villosum (ASP) were investigated. Firstly, the optimal parameters for the extraction of ASP were obtained by using a Box-Behnken Design as follows: extraction temperature 78.8 °C, extraction time 71.3 min and ratio of water to raw material 39.3 ml/g. Then, the crude ASP was sequentially purified by chromatography of DEAE-52 and Sephadex G-100, resulting in three purified fractions of ASP-1, ASP-2 and ASP-3. The results showed that ASP-3 had a larger molecular weight and higher contents of protein, sulfuric radical and uronic acid than the other polysaccharide fractions. Furthermore, ASP-3 showed relative stronger inhibitory activity on the growth of HepG2 cells. Finally, ASP had strong free radical scavenging activities in vitro and significantly prevented the formation of malondialdehyde and enhanced the activities of antioxidant enzymes in CCl4-induced liver injury mice.
在本研究中，对草果种子多糖（ASP）进行了提取、纯化、表征和生物活性研究。首先，采用 Box-Behnken 设计得到了 ASP 提取的最佳参数：提取温度 78.8°C，提取时间 71.3 分钟，水与原料的比例为 39.3ml/g。然后，粗 ASP 依次通过 DEAE-52 和 Sephadex G-100 层析进行纯化，得到三个纯化的 ASP 级分 ASP-1、ASP-2 和 ASP-3。结果表明，ASP-3 的分子量较大，且含有更多的蛋白质、硫酸根和糖醛酸。此外，ASP-3 对 HepG2 细胞的生长具有相对较强的抑制活性。最后，ASP 具有较强的体外自由基清除活性，能显著抑制丙二醛的形成，并增强 CCl4 诱导的肝损伤小鼠抗氧化酶的活性。
