桑（Morus alba L.）叶多糖的提取优化、特性分析及其体外抗氧化活性研究。
Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L.) leaves.
机构信息
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
College of Life Sciences and Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
出版信息
Carbohydr Polym. 2015 Sep 5;128:52-62. doi: 10.1016/j.carbpol.2015.04.028. Epub 2015 Apr 21.
Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry leaves (MLP) were investigated in the present study. The optimal extraction conditions with an extraction yield of 10.0 ± 0.5% for MLP were determined as follows: extraction temperature 92 °C, extraction time 3.5h and ratio (v/w, mL/g) of extraction solvent (water) to raw material 34. Two purified fractions, MLP-3a and MLP-3b with molecular weights of 80.99 and 3.64 kDa, respectively, were obtained from crude MLP by chromatography of DEAE-Cellulose 52 and Sephadex G-100. Fourier transform-infrared spectroscopy revealed that crude MLP, MLP-3a and MLP-3b were acidic polysaccharides. Furthermore, crude MLP and MLP-3a had more complicated monosaccharide compositions, while MLP-3b had a relatively higher content of uronic acid. Crude MLP, MLP-3a and MLP-3b exhibited potent Fe(2+) chelating power and scavenging activities on 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide and 2,2'-azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid) radicals. The results suggested that MLP could be explored as natural antioxidant.
本研究旨在优化桑树叶（MLP）多糖的提取条件，对其进行表征，并研究其体外抗氧化活性。确定了 MLP 最佳提取条件，提取产率为 10.0±0.5%，具体条件为：提取温度 92℃，提取时间 3.5h，提取溶剂（水）与原料的比例（v/w，mL/g）为 34。通过 DEAE-纤维素 52 和 Sephadex G-100 层析，从粗 MLP 中得到两种纯化级分 MLP-3a 和 MLP-3b，分子量分别为 80.99 和 3.64kDa。傅里叶变换红外光谱表明，粗 MLP、MLP-3a 和 MLP-3b 均为酸性多糖。此外，粗 MLP 和 MLP-3a 具有更复杂的单糖组成，而 MLP-3b 则具有更高的糖醛酸含量。粗 MLP、MLP-3a 和 MLP-3b 具有较强的 Fe（2+）螯合能力和对 1,1-二苯基-2-苦基肼、羟基、超氧自由基和 2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）自由基的清除活性。结果表明，MLP 可作为天然抗氧化剂进行开发利用。
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