Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, Jilin 130062, People's Republic of China.
Int Immunopharmacol. 2013 Jun;16(2):313-21. doi: 10.1016/j.intimp.2013.04.010. Epub 2013 Apr 23.
Farrerol, a new type of 2,3-dihydro-flavonoid, has been isolated from the leaves of Rhododendron dauricum L. In the present study, we found that farrerol exerted potent immunosuppressive effects on murine T cells both in vitro and in vivo. In vitro, farrerol markedly suppressed concanavalin A (ConA)-induced lymphocyte proliferation, Th1 and Th2 cytokine production, cluster of differentiation 4-positive (CD4(+)) T cell populations, and the ratio of CD4(+)/cluster of differentiation 8-positive (CD8(+)) T cells. Moreover, farrerol significantly inhibited the T cell-mediated delayed-type hypersensitivity (DTH) reaction in vivo. In addition, we investigated signal transduction mechanisms to determine the effects of farrerol by Western blotting. The data revealed that farrerol could downregulate the activation of the nuclear factor κB (NF-қB) and nuclear factor of activated T cells 2 (NFAT2) signal transduction pathways. These findings suggested that farrerol has potential effects on the regulation of the immune system and could be developed as a practicable immunosuppressive compound.
法呢醇，一种新型的 2,3-二氢黄酮类化合物，已从杜鹃属植物的叶子中分离出来。在本研究中，我们发现法呢醇在体内和体外均对小鼠 T 细胞具有很强的免疫抑制作用。体外实验中，法呢醇显著抑制刀豆蛋白 A（ConA）诱导的淋巴细胞增殖、Th1 和 Th2 细胞因子产生、分化群 4 阳性（CD4(+)）T 细胞群和 CD4(+) /分化群 8 阳性（CD8(+)）T 细胞的比值。此外，法呢醇还显著抑制体内 T 细胞介导的迟发型超敏反应（DTH）反应。此外，我们通过 Western blot 研究了信号转导机制来确定法呢醇的作用。数据表明，法呢醇可以下调核因子 κB（NF-қB）和活化 T 细胞核因子 2（NFAT2）信号转导通路的激活。这些发现表明法呢醇可能对免疫系统的调节有影响，并可能开发为一种可行的免疫抑制剂。
