Guan Shuang, Fang Baochen, Song Bocui, Xiong Ying, Lu Jing
Key Laboratory of Zoonosis, Ministry of Education College of Veterinary Medicine and.
Immunopharmacol Immunotoxicol. 2014 Aug;36(4):290-6. doi: 10.3109/08923973.2014.932798. Epub 2014 Jun 26.
Abstract Alpinetin, a flavonoid compound extracted from the seeds of Alpinia katsumadai Hayata, has been known to possess antibacterial, anti-inflammatory and other important therapeutic activities. In the current study, we investigated alpinetin for its immunosuppressive effect on activation and cytokines secretion of murine T lymphocytes. The data showed that alpinetin markedly suppressed ConA-induced murine splenocyte proliferation, Th1/Th2 cytokines production, CD4(+) T-cell populations and ratio of CD4(+)/CD8(+). This inspired us to further study the effects of alpinetin in vivo. The results showed that administration of alpinetin suppressed T-cell-mediated delayed-type hypersensitivity reaction in mice. In addition, we studied signal transduction pathways about T-cell activation on puried murine T lymphocytes by Western-blot assay. The data revealed that alpinetin could shock the activation of NF-κB, NFAT2 signal transduction pathways. These observations indicated that alpinetin have potential effects in downregulating the immune system and might be developed as a useful immunosuppressive agent in treating undesired immune responses.
摘要 山姜素是从草豆蔻种子中提取的一种黄酮类化合物，已知具有抗菌、抗炎和其他重要治疗活性。在本研究中，我们研究了山姜素对小鼠T淋巴细胞活化和细胞因子分泌的免疫抑制作用。数据显示，山姜素显著抑制刀豆蛋白A诱导的小鼠脾细胞增殖、Th1/Th2细胞因子产生、CD4(+) T细胞群体以及CD4(+)/CD8(+) 比值。这促使我们进一步研究山姜素在体内的作用。结果表明，给予山姜素可抑制小鼠T细胞介导的迟发型超敏反应。此外，我们通过蛋白质免疫印迹法研究了纯化的小鼠T淋巴细胞上T细胞活化的信号转导途径。数据显示，山姜素可抑制NF-κB、NFAT2信号转导途径的活化。这些观察结果表明，山姜素在下调免疫系统方面具有潜在作用，可能被开发为治疗不良免疫反应的有用免疫抑制剂。
