Institute of Food and Nutraceutical Science, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Institute of Food and Nutraceutical Science, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Toxicol In Vitro. 2018 Sep;51:54-62. doi: 10.1016/j.tiv.2018.05.002. Epub 2018 May 4.
This study investigated whether and how 3-chloro-1,2-propanediol (3-MCPD) fatty acid esters, a group of food contaminants formed during processing, might inhibit the immune system through suppressing T lymphocyte activation for the first time. Three 3-MCPD esters including 1-palmitoyl-3-chloropropanediol (1-pal), 2-palmitoyl-3-chloropropanediol (2-pal), and1,2-dipalmitoyl-3-chloropropanediol (dipal) were selected as the probe compounds to test the possible effects of fatty acid structure on their potential immune inhibitory effect. The results showed that 1-pal and 2-pal, but not dipal, significantly suppressed ConA-induced T lymphocyte proliferation, cell cycle activity, Th1 and Th2 cytokine secretion, CD4 T cell populations, and the ratio of CD4/CD8 T cells under the experimental conditions. Moreover, Western blotting and immunofluorescence analyses revealed that 1-pal and 2-pal could inhibit the activation of ConA-stimulated mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathways. In addition, 1-pal significantly suppressed DNFB-induced delayed-type hyper sensitivity (DTH) reaction characterized by the increased ear thickness and IFN-γ production in mice. These observations indicated that 3-MCPD esters exerted a negative effect on T lymphocyte-mediated immunity, and the immunosuppressive activities of 3-MCPD monopalmitates were stronger than 3-MCPD dipalmitate.
本研究首次调查了 3-氯-1,2-丙二醇（3-MCPD）脂肪酸酯这组在加工过程中形成的食品污染物是否以及如何通过抑制 T 淋巴细胞活化来抑制免疫系统。选择三种 3-MCPD 酯，包括 1-棕榈酰基-3-氯丙二醇（1-pal）、2-棕榈酰基-3-氯丙二醇（2-pal）和 1,2-二棕榈酰基-3-氯丙二醇（dipal）作为探针化合物，以测试脂肪酸结构对其潜在免疫抑制作用的可能影响。结果表明，1-pal 和 2-pal，但不是 dipal，在实验条件下显著抑制 ConA 诱导的 T 淋巴细胞增殖、细胞周期活性、Th1 和 Th2 细胞因子分泌、CD4 T 细胞群体以及 CD4/CD8 T 细胞的比例。此外，Western 印迹和免疫荧光分析表明，1-pal 和 2-pal 可以抑制 ConA 刺激的丝裂原活化蛋白激酶（MAPK）和核因子-κB（NF-κB）信号通路的激活。此外，1-pal 显著抑制了二硝基氟苯（DNFB）诱导的迟发型超敏反应（DTH）反应，表现为小鼠耳部厚度增加和 IFN-γ产生增加。这些观察结果表明，3-MCPD 酯对 T 淋巴细胞介导的免疫产生了负面影响，并且 3-MCPD 单棕榈酸酯的免疫抑制活性强于 3-MCPD 二棕榈酸酯。
