Dobrzyńska Małgorzata M, Jankowska-Steifer Ewa A, Tyrkiel Ewa J, Gajowik Aneta, Radzikowska Joanna, Pachocki Krzysztof A
Department of Radiation Hygiene and Radiobiology, National Institute of Public Health-National Institute of Hygiene, Warsaw, Poland.
Environ Toxicol. 2014 Nov;29(11):1301-13. doi: 10.1002/tox.21861. Epub 2013 Apr 25.
Bisphenol A (BPA) is employed in the manufacturing of epoxy, polyester-styrene, and polycarbonate resins, which are used for the production of baby and water bottles and reusable containers, food and beverage packing, dental fillings and sealants. The study was designed to examine the effects of 8-week exposure (a full cycle of spermatogenesis) to BPA alone and in a combination with X-irradiation on the reproductive organs and germ cells of adult and pubescent male mice. Pzh:Sfis male mice were exposed to BPA (5, 10, and 20 mg/kg) or X-rays (0.05 Gy) or to a combination of both (0.05 Gy + 5 mg/kg bw BPA). The following parameters were examined: sperm count, sperm motility, sperm morphology, and DNA damage in male gametes. Both BPA and X-rays alone diminished sperm quality. BPA exposure significantly reduced sperm count in pubescent males compared to adult mice, with degenerative changes detected in seminiferous epithelium. This may suggest a higher susceptibility of germ cells of younger males to BPA action. Combined BPA with X-ray treatment enhanced the harmful effect induced by BPA alone in male germ cells of adult males, whereas low-dose irradiation showed sometimes protective or additive effects in pubescent mice.
双酚A（BPA）用于制造环氧树脂、聚酯 - 苯乙烯树脂和聚碳酸酯树脂，这些树脂用于生产婴儿奶瓶、水瓶和可重复使用的容器、食品和饮料包装、牙科填充物和密封剂。本研究旨在考察单独8周暴露（一个完整的精子发生周期）于双酚A以及双酚A与X射线联合作用对成年和青春期雄性小鼠生殖器官及生殖细胞的影响。将Pzh:Sfis雄性小鼠暴露于双酚A（5、10和20毫克/千克）、X射线（0.05戈瑞）或两者的组合（0.05戈瑞 + 5毫克/千克体重双酚A）。检测了以下参数：精子数量、精子活力、精子形态以及雄配子中的DNA损伤。单独的双酚A和X射线均降低了精子质量。与成年小鼠相比，双酚A暴露显著降低了青春期雄性小鼠的精子数量，在生精上皮中检测到退行性变化。这可能表明年轻雄性的生殖细胞对双酚A的作用更敏感。双酚A与X射线联合处理增强了单独双酚A对成年雄性小鼠生殖细胞的有害作用，而低剂量辐射在青春期小鼠中有时表现出保护或相加作用。
