National Center for Preclinical Reproductive and Genetic Toxicology, National Institute for Research in Reproductive Health, Parel, Mumbai, India.
Reprod Toxicol. 2013 Sep;40:60-8. doi: 10.1016/j.reprotox.2013.05.013. Epub 2013 Jun 13.
The present study investigates the effects of Bisphenol A on the induction of dominant lethal mutation and male reproductive functions. The male rats were gavaged with BPA (10 μg, and 5.0 mg/kg/bw) over a period of six days and control group with vehicle. Each male was cohabited with untreated females sequentially over the period of eight weeks. The mated females were sacrificed on 15th day of gestation. The results revealed a significant increase in dominant lethal mutation rate during fourth and sixth week of mating intervals at 5.0mg/kgbw dose of BPA. These findings demonstrate that mid-spermatids and spermatocytes are more sensitive to BPA exposure. The male rats sacrificed at the end of mating study showed an increase in the sperm DNA damage, and decrease in motility at higher dose. However, significant reductions in sperm production effects were observed at both lower and higher doses of BPA. These preliminary results indicate that BPA may be a weak male germ cell mutagen.
本研究探讨了双酚 A 对显性致死突变和雄性生殖功能的影响。雄性大鼠在六周内通过灌胃给予 BPA（10μg 和 5.0mg/kg/bw），对照组给予载体。每只雄性大鼠在八周的时间内与未经处理的雌性大鼠连续交配。在妊娠第 15 天处死交配后的雌性大鼠。结果显示，在 BPA 5.0mg/kgbw 剂量下，在交配间隔的第四和第六周，显性致死突变率显著增加。这些发现表明，中精母细胞和精母细胞对 BPA 暴露更为敏感。在交配研究结束时处死的雄性大鼠显示精子 DNA 损伤增加，高剂量时运动能力下降。然而，在较低和较高剂量的 BPA 下，均观察到精子产生效应的显著减少。这些初步结果表明，BPA 可能是一种弱雄性生殖细胞诱变剂。
