RNA干扰：针对登革病毒的抗病毒疗法。
RNAi: antiviral therapy against dengue virus.
作者信息
Idrees Sobia, Ashfaq Usman A
机构信息
Department of Bioinformatics and Biotechnology, Government College University (GCU), Faisalabad, Pakistan.
出版信息
Asian Pac J Trop Biomed. 2013 Mar;3(3):232-6. doi: 10.1016/S2221-1691(13)60057-X.
Abstract
Dengue virus infection has become a global threat affecting around 100 countries in the world. Currently, there is no licensed antiviral agent available against dengue. Thus, there is a strong need to develop therapeutic strategies that can tackle this life threatening disease. RNA interference is an important and effective gene silencing process which degrades targeted RNA by a sequence specific process. Several studies have been conducted during the last decade to evaluate the efficiency of siRNA in inhibiting dengue virus replication. This review summarizes siRNAs as a therapeutic approach against dengue virus serotypes and concludes that siRNAs against virus and host genes can be next generation treatment of dengue virus infection.
摘要
登革病毒感染已成为一种全球威胁，影响着世界上约100个国家。目前，尚无针对登革热的获批抗病毒药物。因此，迫切需要开发能够应对这种危及生命疾病的治疗策略。RNA干扰是一种重要且有效的基因沉默过程，它通过序列特异性过程降解靶向RNA。在过去十年中，已经进行了多项研究来评估小干扰RNA（siRNA）抑制登革病毒复制的效率。本综述总结了将siRNA作为针对登革病毒血清型的治疗方法，并得出结论，针对病毒和宿主基因的siRNA可能成为登革病毒感染的下一代治疗方法。
相似文献
1
RNAi: antiviral therapy against dengue virus.RNA干扰：针对登革病毒的抗病毒疗法。
Asian Pac J Trop Biomed. 2013 Mar;3(3):232-6. doi: 10.1016/S2221-1691(13)60057-X.
2
[Study on the inhibitory effect of RNA interference on replication of dengue virus].[RNA干扰对登革病毒复制的抑制作用研究]
Bing Du Xue Bao. 2010 Sep;26(5):373-8.
3
Targeted delivery of small interfering RNA to human dendritic cells to suppress dengue virus infection and associated proinflammatory cytokine production.靶向递送小干扰 RNA 至人树突状细胞以抑制登革病毒感染和相关促炎细胞因子产生。
J Virol. 2010 Mar;84(5):2490-501. doi: 10.1128/JVI.02105-08. Epub 2009 Dec 16.
4
Role of RNA interference (RNAi) in dengue virus replication and identification of NS4B as an RNAi suppressor.RNA 干扰（RNAi）在登革病毒复制中的作用及 NS4B 作为 RNAi 抑制剂的鉴定。
J Virol. 2013 Aug;87(16):8870-83. doi: 10.1128/JVI.02774-12. Epub 2013 Jun 5.
5
Small interference RNA profiling reveals the essential role of human membrane trafficking genes in mediating the infectious entry of dengue virus.小干扰 RNA 谱分析揭示了人类膜转运基因在介导登革热病毒感染进入中的重要作用。
Virol J. 2010 Feb 1;7:24. doi: 10.1186/1743-422X-7-24.
6
RNA interference modulates replication of dengue virus in Drosophila melanogaster cells.RNA 干扰调节登革热病毒在黑腹果蝇细胞中的复制。
BMC Microbiol. 2010 Apr 27;10:127. doi: 10.1186/1471-2180-10-127.
7
Inhibition of dengue virus infection by small interfering RNAs that target highly conserved sequences in the NS4B or NS5 coding regions.靶向NS4B或NS5编码区高度保守序列的小分子干扰RNA对登革病毒感染的抑制作用。
Arch Virol. 2018 May;163(5):1331-1335. doi: 10.1007/s00705-018-3757-2. Epub 2018 Feb 1.
8
Inhibition of dengue virus infections in cell cultures and in AG129 mice by a small interfering RNA targeting a highly conserved sequence.靶向高度保守序列的小干扰 RNA 抑制细胞培养和 AG129 小鼠中的登革热病毒感染。
J Virol. 2011 Oct;85(19):10154-66. doi: 10.1128/JVI.05298-11. Epub 2011 Jul 27.
9
Adenovirus delivered short hairpin RNA targeting a conserved site in the 5' non-translated region inhibits all four serotypes of dengue viruses.腺病毒递送靶向 5'非翻译区保守位点的短发夹 RNA 抑制所有四种血清型登革热病毒。
PLoS Negl Trop Dis. 2012;6(7):e1735. doi: 10.1371/journal.pntd.0001735. Epub 2012 Jul 24.
10
Delivery of antiviral small interfering RNA with gold nanoparticles inhibits dengue virus infection in vitro.金纳米粒子递呈的抗病毒小干扰 RNA 抑制体外登革热病毒感染。
J Gen Virol. 2014 Aug;95(Pt 8):1712-1722. doi: 10.1099/vir.0.066084-0. Epub 2014 May 14.
引用本文的文献
1
Designing siRNAs against non-structural genes of all serotypes of Dengue virus using RNAi technology - A computational investigation.利用RNA干扰技术针对登革病毒所有血清型的非结构基因设计小干扰RNA——一项计算研究。
J Genet Eng Biotechnol. 2025 Sep;23(3):100523. doi: 10.1016/j.jgeb.2025.100523. Epub 2025 Jun 23.
2
Prediction of virus-host interactions and identification of hot spot residues of DENV-2 and SH3 domain interactions.预测病毒-宿主相互作用和鉴定 DENV-2 与 SH3 结构域相互作用的热点残基。
Arch Microbiol. 2024 Mar 14;206(4):162. doi: 10.1007/s00203-024-03892-x.
3
Delivery of siRNAs against MERS-CoV in Vero and HEK-293 cells: A comparative evaluation of transfection reagents.在Vero细胞和HEK - 293细胞中递送针对中东呼吸综合征冠状病毒（MERS-CoV）的小干扰RNA（siRNAs）：转染试剂的比较评估
J King Saud Univ Sci. 2023 Apr;35(3):102540. doi: 10.1016/j.jksus.2023.102540. Epub 2023 Jan 5.
4
Computational Design and Experimental Evaluation of MERS-CoV siRNAs in Selected Cell Lines.中东呼吸综合征冠状病毒（MERS-CoV）小分子干扰RNA（siRNAs）在选定细胞系中的计算设计与实验评估
Diagnostics (Basel). 2023 Jan 2;13(1):151. doi: 10.3390/diagnostics13010151.
5
In Vitro Inhibition of Replication of Dengue Virus Serotypes 1-4 by siRNAs Bound to Non-Toxic Liposomes.非毒性脂质体结合的 siRNA 对登革病毒血清型 1-4 复制的体外抑制作用。
Viruses. 2022 Feb 7;14(2):339. doi: 10.3390/v14020339.
6
Clinical and Molecular facets of Dengue Virus infection from Bengaluru, South India.印度南部班加罗尔登革热病毒感染的临床和分子层面
Nepal J Epidemiol. 2021 Sep 30;11(3):1053-1062. doi: 10.3126/nje.v11i3.37712. eCollection 2021 Sep.
7
Dengue Virus Infection: A Tale of Viral Exploitations and Host Responses.登革热病毒感染：病毒利用与宿主反应的故事。
Viruses. 2021 Sep 30;13(10):1967. doi: 10.3390/v13101967.
8
In Vitro Inhibitory Analysis of Rationally Designed siRNAs against MERS-CoV Replication in Huh7 Cells.针对 MERS-CoV 复制的理性设计 siRNAs 在 Huh7 细胞中的体外抑制分析。
Molecules. 2021 Apr 29;26(9):2610. doi: 10.3390/molecules26092610.
9
Oligonucleotide-Based Approaches to Inhibit Dengue Virus Replication.基于寡核苷酸的抑制登革病毒复制方法。
Molecules. 2021 Feb 11;26(4):956. doi: 10.3390/molecules26040956.
10
prediction and experimental validation of siRNAs targeting ORF1ab of MERS-CoV in Vero cell line.针对中东呼吸综合征冠状病毒（MERS-CoV）ORF1ab的小干扰RNA（siRNAs）在Vero细胞系中的预测及实验验证
Saudi J Biol Sci. 2021 Feb;28(2):1348-1355. doi: 10.1016/j.sjbs.2020.11.066. Epub 2020 Nov 27.
本文引用的文献
1
Adenovirus delivered short hairpin RNA targeting a conserved site in the 5' non-translated region inhibits all four serotypes of dengue viruses.腺病毒递送靶向 5'非翻译区保守位点的短发夹 RNA 抑制所有四种血清型登革热病毒。
PLoS Negl Trop Dis. 2012;6(7):e1735. doi: 10.1371/journal.pntd.0001735. Epub 2012 Jul 24.
2
Bioinformatics prediction of siRNAs as potential antiviral agents against dengue viruses.作为抗登革病毒潜在抗病毒剂的小干扰RNA的生物信息学预测
Bioinformation. 2012;8(11):519-22. doi: 10.6026/97320630008519. Epub 2012 Jun 16.
3
RNAi in the regulation of mammalian viral infections.RNAi 在调控哺乳动物病毒感染中的作用。
BMC Biol. 2012 Jun 26;10:58. doi: 10.1186/1741-7007-10-58.
4
RNA interference mediated inhibition of dengue virus multiplication and entry in HepG2 cells.RNA 干扰介导的登革热病毒在 HepG2 细胞中的增殖和进入抑制。
PLoS One. 2012;7(3):e34060. doi: 10.1371/journal.pone.0034060. Epub 2012 Mar 23.
5
Serotype and genotype analysis of dengue virus by sequencing followed by phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan.通过测序对登革热病毒进行血清型和基因型分析，并利用 2007-2009 年巴基斯坦三次小型暴发的样本进行系统进化分析。
BMC Microbiol. 2011 Sep 10;11:200. doi: 10.1186/1471-2180-11-200.
6
Inhibition of dengue virus infections in cell cultures and in AG129 mice by a small interfering RNA targeting a highly conserved sequence.靶向高度保守序列的小干扰 RNA 抑制细胞培养和 AG129 小鼠中的登革热病毒感染。
J Virol. 2011 Oct;85(19):10154-66. doi: 10.1128/JVI.05298-11. Epub 2011 Jul 27.
7
siRNAs: potential therapeutic agents against hepatitis C virus.siRNAs：针对丙型肝炎病毒的潜在治疗药物。
Virol J. 2011 Jun 6;8:276. doi: 10.1186/1743-422X-8-276.
8
RIG-I, MDA5 and TLR3 synergistically play an important role in restriction of dengue virus infection.RIG-I、MDA5 和 TLR3 协同作用，在限制登革病毒感染方面发挥重要作用。
PLoS Negl Trop Dis. 2011 Jan 4;5(1):e926. doi: 10.1371/journal.pntd.0000926.
9
Best practices in dengue surveillance: a report from the Asia-Pacific and Americas Dengue Prevention Boards.登革热监测最佳实践：来自亚太和美洲登革热预防理事会的报告。
PLoS Negl Trop Dis. 2010 Nov 16;4(11):e890. doi: 10.1371/journal.pntd.0000890.
10
Dengue: a continuing global threat.登革热：持续的全球威胁。
Nat Rev Microbiol. 2010 Dec;8(12 Suppl):S7-16. doi: 10.1038/nrmicro2460.
Zotero 插件