Suppr超能文献

使用粪便采样检测肯尼亚裂谷地区野生水鸟中的禽流感病毒。

Detection of avian influenza viruses in wild waterbirds in the Rift Valley of Kenya using fecal sampling.

机构信息

U.S. Army Medical Research Unit-Kenya, Nairobi, Kenya.

出版信息

Vector Borne Zoonotic Dis. 2013 Jun;13(6):394-400. doi: 10.1089/vbz.2011.0926. Epub 2013 Apr 26.

Abstract

Highly pathogenic avian influenza virus A/H5N1 has been reported in 11 African countries. Migratory waterbirds have the potential of introducing A/H5N1 into east Africa through the Rift Valley of Kenya. We present the results of a wild bird surveillance system for A/H5N1 and other avian influenza viruses based on avian fecal sampling in Kenya. We collected 2630 fecal samples in 2008. Viral RNA was extracted from pools of 3-5 fecal samples and analyzed for presence of avian influenza virus RNA by real-time RT-PCR. Twelve (2.3%) of the 516 sample pools were positive for avian influenza virus RNA, 2 of which were subtyped as H4N6 viruses. This is the first report of avian influenza virus in wild birds in Kenya. This study demonstrates the success of this approach in detecting avian influenza virus in wild birds and represents an efficient surveillance system for avian influenza virus in regions with limited resources.

摘要

高致病性禽流感病毒 A/H5N1 已在 11 个非洲国家报告。候鸟有可能通过肯尼亚大裂谷将 A/H5N1 引入东非。我们介绍了肯尼亚基于鸟类粪便采样的 A/H5N1 和其他禽流感病毒的野生鸟类监测系统的结果。我们在 2008 年收集了 2630 份粪便样本。从 3-5 份粪便样本的混合物中提取病毒 RNA,并通过实时 RT-PCR 分析是否存在禽流感病毒 RNA。516 个样本混合物中有 12 个(2.3%)对禽流感病毒 RNA 呈阳性,其中 2 个被亚分型为 H4N6 病毒。这是肯尼亚野生鸟类中禽流感病毒的首次报告。本研究证明了这种方法在检测野生鸟类中的禽流感病毒方面的成功,并代表了在资源有限的地区进行禽流感病毒监测的有效系统。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验